Keio University · Medicine
Professor Akiyoshi Hirayama's research lab specializes in metabolomics, focusing on the development and application of advanced analytical technologies—particularly capillary electrophoresis-mass spectrometry (CE-MS)—for comprehensive, quantitative profiling of charged metabolites in biological samples. The lab investigates metabolic reprogramming in cancer, such as the Warburg effect, using clinical tissue and blood samples to uncover disease biomarkers and metabolic signatures. A key research direction involves optimizing analytical methods for improved sensitivity and reproducibility in metabolomic studies, especially under real-world pre-analytical conditions. The lab also pioneers innovative interfaces and separation techniques to enhance detection limits and dynamic range for cationic metabolites.
Figures are computed from collected data and may differ slightly.
Most cancer cells predominantly produce energy by glycolysis rather than oxidative phosphorylation via the tricarboxylic acid (TCA) cycle, even in the presence of an adequate oxygen supply (Warburg effect). However, little has been reported regarding the direct measurements of global metabolites in clinical tumor tissues. Here, we applied capillary electrophoresis time-of-flight mass spectrometry, which enables comprehensive and quantitative analysis of charged metabolites, to simultaneously mea
The development of high-throughput metabolite measurement technologies has enabled the use of metabolomics for epidemiologic studies by profiling metabolite concentrations in large cohorts of human blood samples. Standard protocols are necessary to obtain unbiased profiles through multiple runs over long periods of time and to allow reliable statistical analyses. This study assessed the effects of sampling procedures and storage conditions on the stability of metabolomic profiles in plasma and s
Sheath-flow capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a new tool for comprehensive analysis of charged metabolites. However, it needs to be more sensitive. Here, we report a sheathless capillary electrophoresis-electrospray ionization-mass spectrometry method for cationic metabolome analysis. This system used a high-sensitivity porous sprayer interface and 10% (v/v) acetic acid as the background electrolyte (BGE). Under optimized conditions, 53 cationic metabolites, incl
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