Keio University · Biochemistry, Genetics and Molecular Biology
Professor Andrea Fiorani's research lab specializes in electrochemiluminescence (ECL) as a powerful analytical and sensing technique, with a strong focus on advancing electrode materials, luminophores, and reaction mechanisms for enhanced sensitivity and stability. The lab pioneers the use of advanced materials such as boron-doped diamond electrodes to overcome limitations in signal reproducibility and interference, particularly in biological and clinical applications. Key research directions include in situ generation of coreactants (e.g., hydrogen peroxide), surface engineering of electrodes (e.g., polypyrrole-derived carbon films), and the development of chemical lens effects to control the ECL-emitting layer thickness for high-resolution imaging. The lab’s work bridges fundamental electrochemistry with practical applications in biosensing, point-of-care diagnostics, and bioimaging.
Figures are computed from collected data and may differ slightly.
Electrochemiluminescence (ECL) microscopy is an emerging technique with a wide range of imaging applications and unique properties in terms of high spatial resolution, surface confinement and favourable signal-to-noise ratio. Despite its successful analytical applications, tuning the depth of field (<i>i.e.</i>, thickness of the ECL-emitting layer) is a crucial issue. Indeed, the control of the thickness of this ECL region, which can be considered as an "evanescent" reaction layer, limits the de
The electrogenerated chemiluminescence of luminol is a process by which light generation is triggered by adding hydrogen peroxide and then applying a suitable electrode potential. Here, we take this phenomenon one step forward by avoiding the addition of hydrogen peroxide using a smart combination of a boron-doped diamond electrode and a carbonate electrolyte to generate the hydrogen peroxide directly in situ. The reaction occurs because of the carbonate electrochemical oxidation to peroxydicarb
We report on the use of boron doped diamond electrodes for the electrochemiluminescence (ECL) of the coreactant peroxydisulfate and the luminophore ruthenium(II)-tris(2,2'-bipyridine). Compared to common electrode materials (i.e., Pt, Au, glassy carbon), boron doped diamond has a large overpotential for the evolution of hydrogen in aqueous electrolyte solutions. This intrinsic feature enables reductive-oxidation ECL with peroxydisulfate to be obtained without interference from hydrogen evolution
Electrochemiluminescence (ECL) is a powerful transduction technique where light emission from a molecular species is triggered by an electrochemical reaction. Application to biosensors has led to a wide range of electroanalytical methods with particular impact on clinical analysis for diagnostic and therapeutic monitoring. Therefore, the quest for increasing the sensitivity while maintaining reproducible and easy procedures has brought investigations and innovations in (i) electrode materials, (
Abstract Sensors rely on a plurality of transduction methods, and among the available selections, electrochemical techniques offer the peculiar advantage of an easy interfacing between biological recognition elements and electronic signal transduction processes. Furthermore, electrochemical devices are qualified for decentralized point‐of‐care testing as they can be easily downsized and made at low cost for a wide range of potential applications, for example biomedical or environmental. Electroc
Electrogenerated chemiluminescence (ECL) refers to the phenomenon of light emission from molecular species which is triggered by an electrochemical reaction. Therefore, like most electrochemical systems, the electrode material plays a pivotal role and much effort has been made in order to find the best material for ECL, in terms of light signal intensity and long-term stability, especially after the development of ECL for analytical applications. In this article, we will introduce and highlight
Abstract Increasing the light emission of electrogenerated chemiluminescence is an important goal for enhancing the sensitivity for potential practical applications. Electrogenerated chemiluminescence is primarily triggered by a heterogeneous electron transfer reaction, for which the electrode material plays a pivotal role. We investigated how a platinum electrode, one of the most used but poorly efficient noble metal electrode materials in electrogenerated chemiluminescence, can be modified to
In this study, we investigated ammonia synthesis from electrochemical nitrate reduction using a boron-doped diamond (BDD) electrode. Several parameters were optimized, including the boron doping level in BDD, reduction potential, cell type, and electrolyte, to reach an ammonia production rate of 67 ± 12 μmol cm–2 h–1 with a Faradaic efficiency of 98 ± 6%. The ammonia production rate could be enhanced up to 184 μmol cm–2 h–1 by adjusting the boron doping level. From kinetic measurements during am
In this work, we quantify the electrogenerated chemiluminescence arising from the reaction of electrogenerated tris(bipyridine)ruthenium(iii) with hydroxyl ions, in terms of emission intensity and reaction rate. Different electrode materials (glassy carbon and boron-doped diamond) and different supporting electrolytes (perchlorate, phosphate, and carbonate) were investigated with pH variation. Relative quantification of the electrogenerated chemiluminescence was achieved using the Ru(bpy)<sub>3<
A photo-assisted electrochemical system converting CO 2 into formic acid by photoelectrochemical water oxidation at TiO 2 nanotubes coupled with electrochemical CO 2 reduction at boron-doped diamond.
Open papers in the app to read, cite, and organize with AI.
