Tohoku University · Biochemistry, Genetics and Molecular Biology
히데타카 아키타 교수의 연구실은 주로 유전자 치료를 위한 나노입자 기반 약물 전달 시스템을 개발하고 있습니다. 특히, 세포 내 환경(산성 환경과 높은 환원 상태)을 동시에 감지하는 기능성 지질 유사 물질(ssPalm)을 핵심으로 하여, DNA를 효율적으로 세포 내로 전달하고 핵으로 이동시키는 데 중점을 두고 있습니다. 이는 간암 및 난치성 신세포암과 같은 암 치료에 응용 가능한 시스템적 유전자 전달 기술의 개발을 목표로 합니다.
Figures are computed from collected data and may differ slightly.
SS-cleavable proton-activated lipid-like material (ssPalm) functions as a key element in a lipid nanoparticle in which pDNA is encapsulated. The ssPalm contains dual sensing motifs that can respond to the intracellular environment; a proton-sponge unit (tertiary amines) that functions in response to an acidic environment (endosome/lysosome), and disulfide bonding that can be cleaved in a reducing environment (cytosol).
In the present study, the nuclear delivery of a green fluorescence protein (GFP)-encoding pDNA condensed by protamine was investigated in terms of trans-gene expression after cytoplasmic (E(cyt)) and nuclear (E(nuc)) microinjection. To compare the nuclear transfer process, a novel parameter; the nuclear transfer (NT) score was introduced. The E(cyt) value for protamine/pDNA particles increased in a charge ratio-dependent manner. The calculated NT score showed that this increase results from an e
A renal cell carcinoma (RCC) is one of the refractory tumors, since it readily acquires resistance against chemotherapy. Thus, alternative therapeutic approaches such as obstructing the neovasculature are needed. We previously reported on the development of a plasmid DNA (pDNA)-encapsulating liposomal nanoparticle (LNP) as a hepatic gene delivery system that is applicable to systemic administration. The key molecular component is a SS-cleavable and pH-activated lipid-like material (ssPalm) that
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