Hokkaido University · Medicine
Professor Hiroshi Nomoto's research lab specializes in glycobiology and chemoprevention, focusing on the structural characterization of glycoproteins—particularly the carbohydrate structures in ion channels like the acetylcholine receptor—and investigating natural compounds for their protective effects against colorectal carcinogenesis. The lab employs advanced analytical techniques such as NMR spectroscopy and HPLC to elucidate complex oligosaccharide structures, while also exploring the biological impact of polyphenols like proanthocyanidins and EGCG on cellular processes such as proliferation, apoptosis, and immune cell infiltration in preneoplastic models. Their work bridges structural biochemistry with translational cancer prevention strategies.
Figures are computed from collected data and may differ slightly.
The structure of carbohydrates in acetylcholine receptor (AChR) from Torpedo californica is reported. Oligosaccharides released quantitatively from the whole molecule by N-oligosaccharide glycopeptidase digestion were fractionated by thin-layer chromatography and further purified by high-performance liquid chromatography. We show that more than 70% of the total oligosaccharide chains in Torpedo AChR are of the high-mannose type with the structures (Man)8(GlcNAc)2 and (Man)9(GlcNAc)2. The structu
Effects of proanthocyanidin (PA), procyanidin B-2 (B-2), and epigallocatechin gallate (EGCG) on azoxymethane (AOM)-induced colonic preneoplastic aberrant crypt foci (ACF) formation were investigated using F344 rats. The numbers of total ACF in rats treated with 0.002% PA and 0.05% B-2 were significantly decreased compared with the AOM alone group (control). Cell proliferation in the colon, as shown by proliferating cells nuclear antigen (PCNA), was also reduced in those treatments. The single-st
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