Kazuya Nishimura

Automated mitotic detection in time-lapse phase-contrast microscopy provides us much information for cell behavior analysis, and thus several mitosis detection methods have been proposed. However, these methods still have two problems; 1) they cannot detect multiple mitosis events when there are closely placed. 2) they do not consider the annotation gaps, which may occur since the appearances of mitosis cells are very similar before and after the annotated frame. In this paper, we propose a nove

Cell shape analysis is important in biomedical research. Deep learning methods may perform to segment individual cells if they use sufficient training data that the boundary of each cell is annotated. However, it is very time-consuming for preparing such detailed annotation for many cell culture conditions. In this paper, we propose a weakly supervised method that can segment individual cell regions who touch each other with unclear boundaries in dense conditions without the training data for ce

Cell instance segmentation that recognizes each cell boundary is an important task in cell image analysis. While deep learning-based methods have shown promising performances with a certain amount of training data, most of them require full annotations that show the boundary of each cell. Generating the annotation for cell segmentation is time-consuming and human labor. To reduce the annotation cost, we propose a weakly supervised segmentation method using two types of weak labels (one for cell

Automated mitotic detection in time-lapse phasecontrast microscopy provides us much information for cell behavior analysis, and thus several mitosis detection methods have been proposed. However, these methods still have two problems; 1) they cannot detect multiple mitosis events when there are closely placed. 2) they do not consider the annotation gaps, which may occur since the appearances of mitosis cells are very similar before and after the annotated frame. In this paper, we propose a novel

Cell detection is the task of detecting the approximate positions of cell centroids from microscopy images. Recently, convolutional neural network-based approaches have achieved promising performance. However, these methods require a certain amount of annotation for each imaging condition. This annotation is a time-consuming and labor-intensive task. To overcome this problem, we propose a semi-supervised cell-detection method that effectively uses a time-lapse sequence with one labeled image and

We propose a weakly-supervised cell tracking method that can train a convolutional neural network (CNN) by using only the annotation of "cell detection" (i.e., the coordinates of cell positions) without association information, in which cell positions can be easily obtained by nuclear staining. First, we train co-detection CNN that detects cells in successive frames by using weak-labels. Our key assumption is that co-detection CNN implicitly learns association in addition to detection. To obtain

Detection of mitosis events plays an important role in biomedical research. Deep-learning-based mitosis detection methods have achieved outstanding performance with a certain amount of labeled data. However, these methods require annotations for each imaging condition. Collecting labeled data involves time-consuming human labor. In this paper, we propose a mitosis detection method that can be trained with partially annotated sequences. The base idea is to generate a fully labeled dataset from th

Spatial transcriptomics (ST) is a novel technique that simultaneously captures pathological images and gene expression profiling with spatial coordinates. Since ST is closely related to pathological features such as disease subtypes, it may be valuable to augment image representation with pathological information. However, there are no attempts to leverage ST for image recognition (i.e, patch-level classification of subtypes of pathological image.). One of the big challenges is significant batch