The University of Osaka · Materials Science
Professor Mitsuru Akashi's research lab specializes in advanced biomaterials and tissue engineering, focusing on the development of nanoscale films and 3D micro-tissue systems for regenerative medicine and drug screening. The lab pioneers layer-by-layer (LbL) assembly techniques to fabricate biocompatible, functionalized surfaces that guide cell organization and tissue formation, enabling the creation of thick, layered, and vascularized tissues. A key innovation lies in the integration of endothelial cells within engineered tissue constructs to mimic physiological microenvironments, supporting high-throughput, human-relevant disease modeling and personalized medicine applications. The lab also explores stimuli-responsive nanogels and core-corona nanoparticles for biomedical delivery and diagnostic applications.
Figures are computed from collected data and may differ slightly.
A novel cell-accumulation technique has been developed using highly biocompatible nanofilms by layer-by-layer assembly for the rapid construction of thick layered tissues with a well-controlled layer number and thickness. Furthermore, 3D tissues with highly developed blood capillary networks (over 1 cm2 of layered tissues) were also constructed by sandwiching endothelial cells between the layered tissues. Such a simple and rapid methodology would be useful for tissue engineering and drug assessm
Rapid and automatic development of three-dimensional (3D) human micro-tissue chips which integrate over 400 micro-arrays of simplified multilayered structures is carried out using inkjet printing technology. Comprehensive high-throughput assays of liver functions using the simplified 3D liver structures revealed highest functions of a sandwich structure of endothelial cells and hepatocytes. These 3D-human tissue chips may enable “total-human tissue models” for tailor-made drug screening. As a se
Nanostructured gradient gels with unique bending properties due to their deswelling characteristics are presented. These gradient gels are readily fabricated via electrophoresis followed by photo-polymerization, and subsequent silica extraction. Differences in the physical properties between both sides of the gradient gels are the driving force behind the bending of the gels. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2008/adma2
Pack's drauf: Zu Zellmultischichten gelangt man über 6 nm dicke Filme extrazellulärer Matrix (ECM) aus Fibronectin (FN) und Gelatine auf der Oberfläche einer jeden Zellschicht. Die vierlagige Zellenarchitektur ist gut organisiert und frei tragend. Xenogene Doppelschichtarchitekturen wie in menschlichen Blutgefäßen konnten durch Aufbringen der Nanofilme auf Zelloberflächen hergestellt werden.
The surface design and control of substrates with nanometer- or micrometer-sized polymer films are of considerable interest for both fundamental and applied studies in the biomedical field because of the required surface properties. The layer-by-layer (LbL) technique was discovered in 1991 by Decher and co-workers for the fabrication of polymer multilayers constructed mainly through electrostatic interaction. The scope and applicability of this LbL assembly has been extended by introducing molec
This review describes the preparation of core-corona type polymeric nanoparticles and their applications in various technological and biomedical fields. Over the past two decades, we have studied the synthesis and clinical applications of core-corona polymeric nanoparticles composed of hydrophobic polystyrene and hydrophilic macromonomers. These nanoparticles were utilized as catalyst carriers, carriers for oral peptide delivery, virus capture agents, and vaccine carriers, and so on. Moreover, b
N-vinyl-n-butyramide (NVBA), N-vinylisovaleramide (NVIVA), and N-vinyl-n-valeramide (NVVA), which are N-vinylalkylamides with different alkyl groups were synthesized and their solution behavior in a polymeric form was examined. Copolymers of N-vinylisobutyramide (NVIBA) with N-vinylacetamide (NVA), NVIBA with NVVA, and NVVA with NVA were prepared by the solution polymerization to control the LCSTs. The resultant polyNVBA showed a lower critical solution temperature (LCST) sharply at 32°C, but po
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