Wataru Yamazaki

The LAMP assay is a sensitive, rapid and simple tool for the detection of V. parahaemolyticus and will facilitate the surveillance for control of contamination of V. parahaemolyticus in seafood.

A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and

In this paper a derivative-enhanced variable-fidelity surrogate model approach is developed based on a cokriging formulation. In this approach the absolute values of a high-fidelity function as well as the trends obtained by low-fidelity function values are utilized to develop an accurate surrogate model. Derivative information of arbitrary fidelity levels can be also utilized to develop a more accurate surrogate model. The efficiencies of the developed approaches are investigated by analytic fu

The LAMP assay is a sensitive, rapid and simple tool for the detection of CT-producing V. cholerae and will be useful in facilitating the early diagnosis of human V. cholerae infection.

We developed a loop-mediated isothermal amplification (LAMP) assay for the rapid and simple detection of Campylobacter jejuni and Campylobacter coli. The assay provides a specific LAMP product for each of these two species. The assay correctly identified 65 C. jejuni and 45 C. coli strains, but not 75 non-C. jejuni/coli strains. The sensitivity of the LAMP assay for C. jejuni and C. coli in spiked human stool specimens was 5.6 x 10(3) c.f.u. g(-1) (1.4 c.f.u. per test tube) and 4.8 x 10(3) c.f.u

In this paper, gradient/Hessian-enhanced surrogate models have been developed based on Kriging approaches. The gradient/Hessian-enhanced Kriging methods have been developed based on direct and indirect formulations. The efficiencies of these methods are compared by analytical function fitting, aerodynamic data modeling and 2D airfoil drag minimization problems. For the aerodynamic problems, efficient CFD gradient/Hessian calculation methods are utilized that make use of adjoint and automatic dif

We investigated the efficacy of a loop-mediated isothermal amplification (LAMP) assay for detection of chicken meat samples naturally contaminated with Campylobacter jejuni and Campylobacter coli. A total of 144 Preston enrichment broth cultures from chicken meat samples were assessed by using the LAMP assay and conventional culture methods, which consist of a combination of Preston enrichment culturing and plating onto Butzler and modified charcoal cefoperazone deoxycholate agars. Compared with

Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are the major virulence determinants of Vibrio parahaemolyticus. TRH is further differentiated into TRH1 and TRH2 on the basis of genetic and phenotypic differences. We developed a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for sensitive and rapid detection of the tdh, trh1, and trh2 genes of V. parahaemolyticus. The LAMP assay was designed for both combined and individual detection of the tdh,

The new coronavirus infection (COVID-19) is a major public health concern, with a high burden and risk for infection among patients and healthcare workers. Saliva droplets containing SARS-COV-2 are a major vector for COVID-19 infection, making saliva a promising alternative for COVID-19 testing using nasopharyngeal swab samples. To diagnose COVID-19 patients in the field, a point-of-care test (POCT) using saliva was conceptualized. We have developed a simple method for extracting RNA from saliva

An advanced drag-prediction method, the midfield drag-decomposition method, is applied in aerodynamic design optimization problems. The drag-decomposition method decomposes total drag into wave, profile, and induced and spurious drag components, the latter resulting from the effect of numerical diffusion included in computational fluid dynamics results. Hence, the more accurate drag prediction can be achieved by the elimination of the spurious drag component. In this paper, this method is applie

Abstract The accuracy of drag prediction in unstructured mesh CFD solver of TAS (Tohoku University Aerodynamic Simulation) code is discussed using a drag decomposition method. The drag decomposition method decomposes total drag into wave, profile, induced and spurious drag components, the latter resulting from numerical diffusion and errors. The mesh resolution analysis is conducted by the drag decomposition method. The effect of an advanced unstructured mesh scheme of U‐MUSCL reconstruction is

Consistent geometry parameterization of different models used in multidisciplinary optimization is one of the major issues for efficient design optimization. In this research, physics-based free-form deformation and radial-basis-function-based deformation approaches that allow a direct manipulation of an arbitrary object are proposed for efficient geometry manipulation and parameterization in the optimization processes. The developed methods can deform any computational mesh mapped on the deform

In this paper, a biplane-wing/twin-body-fuselage configuration is discussed for advanced supersonic transport. The supersonic-biplane-wing concept has been proposed by Kusunose et al. ( " Supersonic Biplane—A Review ," Progress in Aerospace Sciences , Vol. 47 , No. 1 , 2011 , pp. 53 – 87 ), and a remarkable drag reduction in supersonic flow conditions has been demonstrated in the literatures. Motivated by the wave reduction effect of the supersonic biplane airfoils, a twin-body-fuselage concept