The University of Osaka · Medicine
마츠우라 요시하루 교수의 연구실은 바이러스 유전체의 기능 해석과 바이러스 유전자 조작 기술 개발을 중심으로, 간염 바이러스(HCV)와 코로나19 바이러스(SARS-CoV-2)의 병원성 기전을 분자생물학적·유전자 기법을 통해 규명하고 있습니다. 특히 바이러스 단백질의 세포 내 분포와 기능, 단백질 분해체(예: PA28γ)가 바이러스 병변 형성에 미치는 영향을 중심으로 연구를 진행하고 있으며, 바이러스 기반 유전자 전달 벡터의 개선 및 응용도 함께 탐구하고 있습니다. 이는 바이러스 감염의 기전 규명과 치료 전략 개발에 기여할 잠재력을 지닙니다.
Figures are computed from collected data and may differ slightly.
Hepatitis C virus (HCV) is a major cause of chronic liver disease that frequently leads to steatosis, cirrhosis, and eventually hepatocellular carcinoma (HCC). HCV core protein is not only a component of viral particles but also a multifunctional protein because liver steatosis and HCC are developed in HCV core gene-transgenic (CoreTg) mice. Proteasome activator PA28gamma/REGgamma regulates host and viral proteins such as nuclear hormone receptors and HCV core protein. Here we show that a knocko
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 (COVID-19). Although multiple mutations have been observed in SARS-CoV-2, functional analysis of each mutation of SARS-CoV-2 has been limited by the lack of convenient mutagenesis methods. In this study, we establish a PCR-based, bacterium-free method to generate SARS-CoV-2 infectious clones. Recombinant SARS-CoV-2 could be rescued at high titer with high accuracy a
Although recombinant baculovirus vectors can be an efficient tool for gene transfer into mammalian cells in vitro, gene transduction in vivo has been hampered by the inactivation of baculoviruses by serum complement. Recombinant baculoviruses possessing excess envelope protein gp64 or other viral envelope proteins on the virion surface deliver foreign genes into a variety of mammalian cell lines more efficiently than the unmodified baculovirus. In this study, we examined the efficiency of gene t
Open papers in the app to read, cite, and organize with AI.
