Kyoto University · Biochemistry, Genetics and Molecular Biology
Professor Yoshinobu Takakura's research lab specializes in advanced drug delivery systems, with a focus on macromolecular prodrugs and hydrodynamics-based gene transfer. The lab investigates innovative strategies for targeted lymphatic delivery using polymeric prodrugs, such as dextran-conjugated mitomycin C, to enhance drug retention and therapeutic efficacy. Another key research direction involves understanding the mechanisms of non-viral gene delivery, particularly the cytosolic delivery of plasmid DNA through transient membrane permeabilization. The lab's work bridges pharmaceutical sciences and translational medicine, aiming to improve the precision and safety of drug and gene therapies.
Figures are computed from collected data and may differ slightly.
These findings suggest that the mechanism for the hydrodynamics-based gene transfer would involve in part the direct cytosolic delivery of pDNA through the cell membrane due to transiently increased permeability.
Absorption and lymphatic transfer of a polymeric prodrug of mitomycin C (MMC), mitomycin C-dextran conjugate (MMC-D), following i.m. injection were studied in rats in order to assess the feasibility of a macromolecular prodrug as a lymphotropic delivery system. Three types of MMC-D, conjugates with dextran with molecular weights of 10,000, 70,000 and 500,000, were synthesized, and the disposition of MMC was determined by bioassay. Following i.m. injection of MMC-D, MMC was retained at the inject
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