Sunghoon Kwon

A rapid antibiotic susceptibility test (AST) is desperately needed in clinical settings for fast and appropriate antibiotic administration. Traditional ASTs, which rely on cell culture, are not suitable for urgent cases of bacterial infection and antibiotic resistance owing to their relatively long test times. We describe a novel AST called single-cell morphological analysis (SCMA) that can determine antimicrobial susceptibility by automatically analyzing and categorizing morphological changes i

Sepsis is one of the major causes of death in the US, necessitating rapid treatment with proper antibiotics. Conventional systems for antibiotic susceptibility testing (AST) take far too long (16-24 h) for the timely treatment of sepsis. This is because they rely on measuring optical density, which relates to bacterial growth, to determine the minimal inhibitory concentrations (MICs) of relevant antibiotics. Thus, there is a desperate need for more improved and rapid AST (RAST) systems. The RAST

DNA-based data storage has emerged as a promising method to satisfy the exponentially increasing demand for information storage. However, practical implementation of DNA-based data storage remains a challenge because of the high cost of data writing through DNA synthesis. Here, we propose the use of degenerate bases as encoding characters in addition to A, C, G, and T, which augments the amount of data that can be stored per length of DNA sequence designed (information capacity) and lowering the

We have designed, fabricated, and demonstrated large vertical displacement vertical microlens scanners with low (<10 V) driving voltage using silicon-on-insulator technology. The unique isolated and pre-engaged vertical comb-drive sets and the coupled-torsion flexure design provide both upward and downward piston motions, as well as low driving voltages. Single-directional devices demonstrate maximum static downward displacement of 8 μm at 10 V/sub dc/. Bidirectional devices demonstrate vertical

We have developed a miniature scanning confocal microscope that uses electrostatically actuated microlenses for focusing and scanning. Objective lenses, scanners, a pupil, and a pinhole of the confocal microscope are microfabricated and integrated into a volume smaller than 2 mm3 by stacking these components. Objective lenses are composed of two vertically cascaded polymer microlenses integrated into micromachined comb actuators. Raster scanning is implemented by electrostatically actuating each

Dynamic synthesis of magnetoresponsive structural-colored microspheres is demonstrated by combining an optofluidic approach with a magnetic property tuning method. The strategy allows for the synthesis of magnetochromatic microspheres with controlled and heterogeneous optical properties in a single synthesis environment. Orientation-dependent color diffraction of the magnetochromatic microspheres is utilized to form structural color patterns using a patterned magnet. Detailed facts of importance

A method for high-throughput 3D self-assembly of 2D photopatterned microstructures using railed microfluidics is presented. Vertical device patterning of heterogeneous materials requires high-level integration using conventional microelectromechanical system (MEMS) technology; however, 3D railed assembly enables easy and fast self-assembly via a fluidic axis-translation process and simple material exchange in microfluidic channels. Individually photopatterned 2D microstructures are axis-translat

We introduce a backside island isolation method for silicon-on-insulator (SOI)-based microelectromechanical systems technology and demonstrate vertical comb drive-based two-dimensional gimbaled micromirrors with large static rotation using the isolation method. The proposed isolation method provides electrical isolation and mechanical coupling of SOI structures without additional dielectric backfill and planarization by utilizing timed etched backside handle wafer structures. The backside island

Shape-encoded silica microparticles for use in multiplexed bioassays were fabricated by using optofluidic maskless lithography (OFML) and tetraethylorthosilicate (TEOS) polymerization. These encoded silica microparticles exhibit excellent bioconjugation properties and negligible non-specific analyte adsorption. Encoded silica microparticles could be useful in a wide variety of applications, including DNA- and protein-based diagnostics.

A magnetically tunable chromatic nanocomposite microactuator is proposed, which utilizes the optical and magnetic behaviors of self-assembled super-paramagnetic nanoparticles fixed in a polymeric microstructure. The original color can be programmed during a simple photolithography process, and the color can be changed just by applying and changing an external magnetic field. These microactuators are capable of acting as pixels in a color-changing pattern. As a service to our authors and readers,

We present a simple and high-throughput method for fabricating free-floating hydrogel cell microcarriers using single exposure UV patterning. We also demonstrate magnetic manipulation of the free-floating cell microcarriers using a magnetic nanoparticle-embedded structure for an active agitation and a solution exchange.

Single cell analysis of heterogeneous circulating tumor cells (CTCs), by which the genomic profiles of rare single CTCs are connected to the clinical status of cancer patients, is crucial for understanding cancer metastasis and the clinical impact on patients. However, the heterogeneity in genotypes and phenotypes and rarity of CTCs have limited extensive single CTC genome research, further hindering clinical investigation. Despite recent efforts to build platforms that separate CTCs, the invest

Although droplet microfludics is a promising technology for handling a number of liquids of a single type of analyte, it has limitations in handling thousands of different types of analytes for multiplex assay. Here, we present a novel "liquid-capped encoded microcapsule", which is applicable to various liquid format assays. Various liquid drops can be graphically encoded and arrayed without repeated dispensing processes, evaporation, and the risk of cross-contamination. Millions of nanoliter-sc