Kyushu University · Biochemistry, Genetics and Molecular Biology
Professor Yoshiko Miura's research lab specializes in glycopolymer nanobiotechnology, focusing on the design and synthesis of synthetic glycopolymers that mimic natural cell-surface saccharides. Her group explores the multivalent interactions between glycopolymers and biological targets such as lectins, pathogens, and amyloid proteins, with applications in drug delivery, tissue engineering, and neurodegenerative disease intervention. Key research directions include the development of chemoenzymatic synthesis methods for glycoconjugate polymers, the engineering of functional nanomaterials like glycopolymer-coated gold nanoparticles, and the investigation of structure–activity relationships in saccharide-protein interactions. The lab emphasizes both fundamental understanding and practical biomedical applications of these biomimetic materials.
Figures are computed from collected data and may differ slightly.
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTGlycopolymer NanobiotechnologyYoshiko Miura*, Yu Hoshino, and Hirokazu SetoView Author Information Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan*E-mail: [email protected]. Tel: +81-92-802-2749.Cite this: Chem. Rev. 2016, 116, 4, 1673–1692Publication Date (Web):October 28, 2015Publication History Received27 April 2015Published online28 October 2015Published inissue 24
Abstract Saccharides on the cell surfaces participate in a number of biochemical phenomena via the protein–saccharide interaction. Synthetic glycopolymers mimic the function of the cell‐surface saccharides and efficiently interact with proteins, cells, and pathogens based on the multivalent effect. Since the biological functions of saccharides are paid much attention, the glycopolymers are being increasingly explored as biomaterials for medicinal application and tissue engineering. This review p
Several hydrophobic α-helical peptides containing a disulfide group were synthesized, and the formation of oriented self-assembled monolayers (SAMs) on gold surface was investigated. The orientation of helices in the SAMs was determined by Fourier transform infrared reflection−absorption spectroscopy measurements. The tilt angle of the helix axis from the surface normal was sensitively affected by the choice of solvent used in the preparation of SAMs, the nature of component amino acids, the mol
Glycopolymers carrying sulfated saccharides with modest sugar contents (11% and 28%) were found to suppress the formation of amyloid fibrils by amyloid beta peptides (Abeta(1-42), Abeta(1-40), and Abeta(25-35)), as evaluated by thioflavin T assays and atomic force microscopy observation. Circular dichroism spectra showed that the conformation of amyloid beta peptides depended on the glycopolymer additives, and that the glycopolymer additives reduced the beta-sheet contents. Neutralization activi
Glycoconjugate polymers with poly(vinyl alcohol) (PVA) backbone were synthesized via a chemoenzymatic method. The sugar alcohols of maltose and lactose were submitted to transesterification in the presence of lipases. The esterification was achieved with high selectivity and yield, and the resulting maltitol and lactitol 6-vinyl sebacates were polymerized by a conventional radical initiator with hydrogen peroxide and ascorbic acid. The glycoconjugate polymers carrying alpha-glucose and beta-gala
Glycopolymers of polyacrylamide derivatives with mannose were prepared via the living radical polymerization of a reversible addition–fragmentation chain transfer reagent. The polymers obtained showed narrow polydispersities. The polymer terminal group was reduced to a thiol, and the resulting polymers were mixed with gold nanoparticles to prepare glycopolymer-substituted gold nanoparticles. The mannose density was adjusted by varying the copolymer preparation and the glycopolymer–polyacrylamide
Abstract Glycopolymer‐substituted gold nanoparticles were prepared via living radical polymerization with a reversible addition‐fragmentation chain transfer (RAFT) reagent. Polyacrylamide derivatives with α‐mannose (α‐Man) and N ‐acetyl‐β‐glucosamine (β‐GlcNAc) were synthesized and hydrogenated to obtain thiol‐terminated polymer. The thiol‐terminated glycopolymers were mixed with gold nanoparticles to yield the polymer substituted gold nanoparticles with various diameters, which aggregated on ad
The arrangement of sugars in glycopolymers contributes to their recognition. The molecular recognition of proteins was controlled by the living radical polymerization of glycopolymers. The glycopolymers were prepared by the copolymerization of propargyl methacrylate (Pr-MA) and triethyleneglycol methacrylate (TEG-MA) via living radical polymerization with a reversible addition-fragmentation glycopolymer chain transfer (RAFT) reagent and by subsequent sugar conjugation by click chemistry. The blo
Hydrophobic helical hexadecapeptides having an ammonium group were investigated on the formation of an oriented thin layer of the helical peptides on a gold surface. The helical peptides in an ethanol solution were incubated with self-assembled monolayers (SAMs) of carboxylate-terminated alkanethiol on a gold surface to form a helical peptide layer. The thickness of the peptide layer and the orientation of the peptide layer were investigated by the surface plasmon resonance and the FTIR reflecti
Abstract A well‐defined glycopolymer was synthesized to investigate its properties. The glycopolymer was obtained with a narrow polydispersity by nitroxide‐mediated radical polymerization of styrene carrying acetylated lactose and by the subsequent deprotection. The cylindrical structure and helical conformation of the polymer were measured by circular dichroism (CD) spectra. The affinities of the polymers towards lectins depended on the degree of polymerization (DP), and the polymers with highe
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