Yoori Choi

Guard cell protoplasts of Vicia faba treated with 10 [mu]M (+)abscisic acid (ABA) in the light exhibited a 20% decrease in diameter within 1.5 h, from 24.1 to 19.6 [mu]m. Within 10 s of administration of ABA, a 90% increase in levels of inositol 1,4,5-trisphosphate was observed, provided that cells were treated with Li+, an inhibitor of inositol phosphatase activity, prior to incubation. Concomitantly, levels of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phospha

Drainage of parenchymal waste through the lymphatic system maintains brain homeostasis. Age-related changes of glymphatic-lymphatic clearance lead to the accumulation beta-amyloid (Aβ) in dementia models. In this study, focused ultrasound treatment in combination with microbubbles (FUS-MB) improved Aβ drainage in early dementia model mice, 5XFAD. FUS-MB enhanced solute Aβ clearance from brain, but not plaques, to cerebrospinal fluid (CSF) space and then deep cervical lymph node (dCLN). dCLN liga

BACKGROUND: Neural stem cells (NSCs) have the ability to generate a variety of functional neural cell types and have a high potential for neuronal cell regeneration and recovery. Thus, they been recognized as the best source of cell therapy for neurodegenerative diseases, such as Parkinson's disease (PD). Owing to the possibility of paracrine effect-based therapeutic mechanisms and easier clinical accessibility, extracellular vesicles (EVs), which possess very similar bio-functional components f

The AICD (APP intracellular Domain) and C31, caspase-cleaved C-terminal fragment of APP, have been found in Alzheimer's disease (AD) patients' brains and have been reported to induce apoptosis in neuronal cells. In recent, the C-terminal fragments of amyloid precursor protein (APP-CTs) have been reported to form a complex with Fe65 and the histone acetyltransferase Tip60 and are thought to be involved in gene transcription. In this study, based on the hypothesis that APP-CTs might exert neurotox

Neuritin, also known as CPG15, is a neurotrophic factor that was initially discovered in a screen to identify genes involved in activity-dependent synaptic plasticity. Neuritin plays multiple roles in the process of neural development and synaptic plasticity, although its binding receptor(s) and downstream signaling effectors remain unclear. In this study, we found that the cortical and hippocampal expression of neuritin is reduced in the brains of Alzheimer's disease (AD) patients and demonstra

Abstract The molecular changes that occur with the progression of Alzheimer’s disease (AD) are well known, but an understanding of the spatiotemporal heterogeneity of changes in the brain is lacking. Here, we investigated the spatially resolved transcriptome in a 5XFAD AD model at different ages to understand regional changes at the molecular level. Spatially resolved transcriptomic data were obtained from 5XFAD AD models and age-matched control mice. Differentially expressed genes were identifi

Abstract Drainage of parenchymal waste through the lymphatic system maintains brain homeostasis. Age-related changes of glymphatic-lymphatic clearance lead to the accumulation beta-amyloid (Aβ) in dementia models. In this study, focused ultrasound treatment in combination with microbubbles (FUS-MB) improved Aβ drainage in early dementia model mice, 5XFAD. FUS-MB enhanced solute Aβ clearance from brain, but not plaques, to cerebrospinal fluid (CSF) space and then deep cervical lymph node (dCLN).

MicroRNAs (miRNAs) fine-tune target protein synthesis by suppressing gene expression, temporally changing along development and possibly in pathological conditions. A method to monitor the action of miRNAs in vivo shall help understand their dynamic behavior during development. In this study, we established a transgenic mouse harboring miR-124 responsive element in their luciferase-eGFP reporter transgenes which enabled monitoring the action of miR-124 in the brain and other organs in vivo by th

본 연구는 기존의 교육과정 자율화 논의에 가정된 교사 자율성에 대한 이해를 비판적으로 고찰하고, 교사 자율성의 개념을 Foucault의 ‘자기 배려’ 논의에 기초하여 새롭게 이해하는 것을 목적으로 한다. 기존의 교육과정 자율화 논의에서 교사 자율성은 국가에 의해 ‘허용’되고 ‘부여’된 것, 특정 내용과 범위로 ‘규범화’된 것, 교사의 ‘외부에서 정당화’된 것으로 이해되어왔다. Foucault의 ‘자기 배려’ 논의는 오늘날 교육과정 자율화의 흐름 속에서 외부 규약으로 인해 제한된, 혹은 그러한 규약으로부터 완전하게 해방된 양극단의 자율성을 부정하고, 교사의 자율적 행위의 가능성을 새롭게 이해할 수 있는 단초를 제공한다. 이러한 Foucault의 논의를 기초로 하여, 본 연구에서는 교사 자율성을 자기 내면에 몰두함으로써 ‘실현’되고 ‘성취’되는 것, 끊임없는 자기 변형을 통한 ‘개별화’된 ‘과정’인 것, ‘존재의 미학’과 ‘윤리적인 자기 창조’로서의 가치를 지닌 것으로 재개념화하고자 하

ABSTRACT The pathophysiological changes that occur with the progression of Alzheimer’s disease (AD) are well known, but understanding the spatiotemporal heterogeneity of the brain is needed. Here, we investigated the spatially resolved transcriptome in a 5XFAD AD model of different ages to understand regional changes at the molecular level. We identified early alterations in the white matter (WM) of the AD model before the definite accumulation of amyloid plaques in the gray matter (GM). Changes

지식 생산의 물질적, 존재론적 전환을 요청하는 신유물론과 이러한 요청에 대응하고자 하는 연구 실천으로서 포스트 질적 탐구는 교육 연구에 있어 질적 연구의 가능성을 다시 그려내기 위한 장치로서 기능할 수 있다. 그러나 교육 연구에서 실제 연구자의 연구 경험을 바탕으로 이들의 가능성을 구체적으로 탐색하려는 시도는 비교적 미흡하다. 이 연구의 목적은 신유물론과 함께 이루어진 질적 연구의 수행 과정에서 연구자가 마주한 포스트 질적 탐구의 가능성을 탐색하는 것이다. 이를 위해, 교사와 교육 실천에 대한 연구자의 질적 연구 경험이 활용되었다. 도나 해러웨이와 캐런 바라드를 중심으로 신유물론과 포스트 질적 탐구가 논의되었으며, 이들과 함께 교사와 교육 실천을 연구하는 과정에서 ‘데이터 생성’, ‘데이터 분석’, ‘연구자 되기’가 어떻게 다르게 실행될 수 있었는지를 해명하였다. 이를 바탕으로, 교육 연구에서 포스트 질적 탐구의 가능성이 다음과 같이 논의되었다. 첫째, 포스트 질적 탐구는 ‘교육 실

Reactivation of the cell cycle, including DNA replication, might play a major role in Alzheimer's disease. RT-PCR, western blotting. we report that the expressions of Swedish double mutation of amyloid precursor protein (Swe-APP) or of the APP intracellular domain (AICD) into NGF-differentiated PC 12 cells or rat primary cortical neurons increased mRNA and protein levels of cyclin D1 and cyclin B1. The treatment with lithium chloride (a glycogen synthase kinase-3β inhibitor), downregulated cycli