The University of Osaka · Engineering
Professor Eiichi Tamiya's research lab specializes in the development of advanced label-free optical biosensors for rapid, sensitive, and real-time detection of biomolecules, with a focus on medical diagnostics and environmental monitoring. The lab pioneers innovative platforms based on localized surface plasmon resonance (LSPR) using gold-capped nanoparticle substrates functionalized with biomolecular probes such as peptide nucleic acids (PNAs) and antibodies. Key research directions include the integration of these biosensors with microfluidic systems for point-of-care diagnostics, particularly for viral pathogens and food allergens. The lab emphasizes practical, low-cost, and high-throughput sensing solutions compatible with standard laboratory equipment like UV–Vis spectrophotometers or flatbed scanners.
Figures are computed from collected data and may differ slightly.
The development of label-free optical biosensors for DNA and other biomolecules has the potential to impact life sciences as well as screening in medical and environmental applications. In this report, we developed a localized surface plasmon resonance (LSPR) based label-free optical biosensor based on a gold-capped nanoparticle layer substrate immobilized with peptide nucleic acids (PNAs). PNA probe was designed to recognize the target DNA related to tumor necrosis factor. The nanoparticle laye
AbstractIn this research, a localized surface plasmon resonance (LSPR) immunosensor based on gold-capped nanoparticle substrate for detecting casein, one of the most potent allergens in milk, was developed. The fabrication of the gold-capped nanoparticle substrate involved a surface-modified silica nanoparticle layer (core) on the slide glass substrate between bottom and top gold layers (shell). The absorbance peak of the gold-capped nanoparticle substrate was observed at ∼520 nm. In addition, t
In recent years, label-free biosensors not requiring external modifications have been receiving intense attention. A label-free optical biosensor, which retains many of the desirable features of conventional surface plasmon resonance (SPR) reflectometry, namely, the ability to monitor the kinetics of biomolecular interactions in real-time without a label has been developed with several important advantages: the biosensor device is easy to fabricate, and simple to implement, requiring only an UV–
Rapid and definitive diagnosis is critical to the prevention of the spread of endemic human pathogenic viruses. Detection of variant specific genes by reverse transcription polymerase chain reaction (RT-PCR) has become a routine diagnostic test for accurate subtyping of RNA viruses, such as influenza. In this paper, we demonstrate the use of a continuous-flow polydimethylsiloxane (PDMS) microfluidic RT-PCR chip and disposable electrical printed (DEP) chips for rapid amplification and sensing of
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