The University of Osaka · Biochemistry, Genetics and Molecular Biology
Professor Fuminori Sakurai's research lab focuses on viral vector development for gene therapy and oncolytic virotherapy, with a particular emphasis on adenovirus and reovirus systems. The lab investigates viral entry mechanisms, host-virus interactions, and immune modulation to enhance therapeutic efficacy. A key direction involves utilizing human induced-pluripotent stem cell-derived hepatocyte-like cells as physiologically relevant models for studying hepatitis B virus infection and antiviral drug development. The lab also explores how oncolytic viruses can overcome immunosuppressive tumor microenvironments by targeting myeloid-derived suppressor cells.
Figures are computed from collected data and may differ slightly.
We have recently developed a replication-defective, recombinant adenovirus (Ad) vector composed of the whole Ad serotype 35 (Ad35), a member of subgroup B. We describe herein the in vitro and in vivo gene transfer properties of Ad35 vector in comparison with Ad serotype 5 (Ad5) and the Ad5F35 vector, which is a fiber-substituted Ad5 vector containing Ad35 fiber proteins. In vitro, Ad35 vector efficiently transduced not only human CAR-positive cells but also CAR-negative cells. Following intraven
In order to understand the life cycle of hepatitis B virus (HBV) and to develop efficient anti-HBV drugs, a useful in vitro cell culture system which allows HBV infection and recapitulates virus-host interactions is essential; however, pre-existing in vitro HBV infection models are often problematic. Here, we examined the potential of human induced-pluripotent stem (iPS) cell-derived hepatocyte-like cells (iPS-HLCs) as an in vitro HBV infection model. Expression levels of several genes involved
Oncolytic reovirus, which possesses 10 segments of dsRNA genome, mediates antitumor effects via not only virus replication in a tumor cell-specific manner, but also activation of antitumor immunity; however, the mechanism(s) of reovirus-induced activation of antitumor immunity have not been fully elucidated. Recent studies have demonstrated that overcoming an immunosuppressive environment in tumor-bearing hosts is important to achieve efficient activation of antitumor immunity. Among the various
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