Keio University · Medicine
Professor Hideyuki Hayashi's research lab specializes in translational cancer genomics and precision oncology, focusing on identifying and validating molecular biomarkers for cancer prognosis and treatment selection. The lab develops and implements next-generation sequencing (NGS)-based genomic profiling platforms to detect actionable gene alterations, microsatellite instability (MSI-H), and mismatch repair deficiency (dMMR) across various solid tumors. A key emphasis is on the clinical application of molecular diagnostics, including the comparison of PCR-based and NGS methods for MSI/dMMR detection, and optimizing chemotherapy regimens such as FOLFIRINOX for Japanese pancreatic cancer patients. The lab also explores the structural and functional mechanisms of enzymes involved in metabolic pathways, as demonstrated by studies on aspartate aminotransferase and its catalytic intermediates.
Figures are computed from collected data and may differ slightly.
The number of mutated driver genes assessed using a targeted deep sequencing assay was a promising prognostic biomarker for pancreatic cancer.
Various malignancies exhibit high microsatellite instability (MSI-H) or mismatch repair deficiency (dMMR). The MSI-IVD kit, a polymerase chain reaction (PCR)-based method, was the first tumor-agnostic companion diagnostic to detect MSI status in MSI-H solid tumors. Recently, next-generation sequencing (NGS), which can also detect MSI-H/dMMR, has been made clinically available; however, its real-world concordance with PCR-based testing of MSI-H/dMMR remains to be investigated. The co-primary end
Precision medicine is a promising strategy for cancer treatment. In this study, we developed an in-house clinical sequencing system to perform a comprehensive cancer genomic profiling test as a clinical examination and analyzed the utility of this system. Genomic DNA was extracted from tumor tissues and peripheral blood cells collected from 161 patients with different stages and types of cancer. A comprehensive targeted amplicon exome sequencing for 160 cancer-related genes was performed using n
When carefully managed, FOLFIRINOX is acceptably safe and efficacious in Japanese patients with unresectable pancreatic cancer.
Journal Article Multiple fixed drug eruption caused by acetaminophen Get access H. Hayashi, H. Hayashi Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita‐ku, Sapporo 060‐8638, Japan Search for other works by this author on: Oxford Academic Google Scholar T. Shimizu, T. Shimizu Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita‐ku, Sapporo 060‐8638, Japan Search for other works by this author on: Oxford Academic Google Scholar H. Shimizu
The reaction of Escherichia coli aspartate aminotransferase (AspAT) with L-erythro-3-hydroxyaspartate (HOAsp) produces an intense absorption at 494 nm (epsilon = 13,650 M-1 cm-1), which is ascribed to the quinonoid intermediate. However, when Tyr70 of AspAT has been replaced by Phe, the enzyme shows only a faint absorption at 494 nm (epsilon = 522 M-1 cm-1) on the reaction with HOAsp. This indicates the involvement of the hydroxy group of Tyr70 in stabilizing the quinonoid intermediate formed fr
Journal Article Epidermotropic metastatic malignant melanoma with a pedunculated appearance Get access H. Hayashi, H. Hayashi Department of Dermatology, Hokkaido University Graduate School of Medicine, N15, W7, Sapporo 060‐8638, Japan Search for other works by this author on: Oxford Academic Google Scholar T. Kawashima, T. Kawashima Department of Dermatology, Hokkaido University Graduate School of Medicine, N15, W7, Sapporo 060‐8638, Japan Search for other works by this author on: Oxford Academi
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