Korea University · Biochemistry, Genetics and Molecular Biology
Professor Jeong-Hoon Kim's research lab focuses on molecular mechanisms of disease-related proteins, particularly in DNA repair and ubiquitin-mediated regulation, as well as applying computational and molecular tools to address pressing ecological and agricultural challenges. The lab investigates the functional characterization of E3 ubiquitin ligases like hFBH1 and explores antimicrobial resistance (AMR) surveillance using machine learning to predict pathogen burden in food animal systems. Additionally, the lab contributes to polar ecology by studying penguin diets and microbiomes through molecular techniques such as metabarcoding, qPCR, and stable isotope analysis, providing insights into Antarctic ecosystem dynamics. The integration of molecular biology, bioinformatics, and environmental science defines the lab’s interdisciplinary approach.
Figures are computed from collected data and may differ slightly.
The positive link between the SWI/SNF and the Gcn5 histone acetyltransferase in transcriptional activation has been well described. Here we report an inhibitory role for Gcn5 in SWI/SNF targeting. We demonstrate that Gcn5-containing complexes directly acetylate the Snf2 subunit of the SWI/SNF complex in vitro, as well as in vivo. Moreover, the acetylation of Snf2 facilitates the dissociation of the SWI/SNF complex from acetylated histones, and reduces its association with promoters in vivo. Thes
Repeated exposure to amphetamine (AMPH) leads to the development of behavioural sensitization that can be demonstrated in rats as enhanced locomotor responding to and self-administration of the drug. Glutamate systems are known to participate in the induction and expression of sensitization by psychostimulants. Group II metabotropic glutamate receptors (mGluRs), because they negatively regulate both vesicular and nonvesicular glutamate release, are thus well positioned to gate its expression. He
Methylation of several lysine residues of histones is a crucial mechanism for relatively long-term regulation of genomic activity. Recent molecular biological studies have demonstrated that the function of histone methylation is more diverse and complex than previously thought. Moreover, studies using newly available genomics techniques, such as exome sequencing, have identified an increasing number of histone lysine methylation-related genes as intellectual disability-associated genes, which hi
The contribution of metabotropic glutamate receptors (mGluRs) in the ventral tegmental area (VTA) to the induction of locomotor sensitization by amphetamine (AMPH) was investigated. Rats pre-exposed to AMPH in the VTA showed significantly higher locomotor activity when subsequently tested with a systemic AMPH challenge than rats preexposed to VTA saline. Rats pre-exposed to AMPH but co-injected with the selective mGluR antagonist (RS)-alpha-methyl-4-carboxyphenylglycine [(RS)-MCPG] did not show
The aryl hydrocarbon receptor (AHR) and AHR nuclear translocator (ARNT) are DNA binding transcription factors with basic helix-loop-helix/Per-Arnt-Sim (bHLH-PAS) domains. These two proteins form a heterodimer that mediates the toxic and biological effects of the environmental contaminant and AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin. The coiled-coil protein coiled-coil coactivator (Co-CoA) is a secondary coactivator for nuclear receptors and enhances nuclear receptor function by interacting
The role of the biologically active CART 55-102 peptide in the nucleus accumbens (NAcc) in the expression of cocaine-induced behavioral sensitization was investigated. Rats were divided into four groups: one for saline and the other three for cocaine pre-exposures (15 mg/kg, i.p., once daily for 7 days). After 3 weeks of withdrawal, rats were microinjected into the NAcc either saline or CART 55-102 (1.0, or 2.5 microg/0.5 microl/side) followed by cocaine challenge (10 mg/kg, i.p.). Microinjectio
In our previous study, we found that a human F-box DNA helicase, named hFBH1, interacted with SKP1 to form an SCF (SKP1-Cul1-F-box protein) complex together with CUL1 and ROC1 in an F-box-dependent manner. The complex immunoprecipitated from crude cell extracts catalyzed polyubiquitin formation in the presence of the ubiquitin-activating and ubiquitin-conjugating enzymes, E1 and E2, respectively. In this report, we characterized the enzymatic properties of the recombinant SCF(hFBH1) complex puri
The role of nucleus accumbens (NAcc) glutamate (GLU) and D(1) dopamine (DA) receptor activation in the expression of locomotor sensitization to amphetamine (AMPH) was investigated in rats. Rats were preexposed to either AMPH or saline, and 2 weeks later their locomotion was assessed after a microinjection into the NAcc of the selective glutamate reuptake blocker l-trans-pyrrolidine-2,4-dicarboxylic acid (PDC) (10 nmol per side), the D(1)-like DA receptor agonists SKF82958 (2.4 nmol per side) and
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