Kenji Sudo

Podophyllotoxin and its derivatives were examined for inhibitory effects on the replication of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), including acyclovir-resistant virus and clinical isolates. Deoxypodophyllotoxin (RD4-6266) proved to be a highly potent and selective inhibitor of all HSV strains in MRC-5 cells. EC50 values of RD4-6283 (in which the methylenedioxy ring A is modified) for HSV-1 and -2 were inferior to those of deoxypodophyllotoxin. However, podorhizol (RD4-6277) and

Although Southeast Asia is a hotspot of global seagrass diversity, there are considerable information gaps in the distribution of seagrass beds. Broad-scale seagrass distribution has not been updated in the global seagrass database by UNEP-WCMC since 2000, although studies on seagrasses have been undertaken intensively in each region. Here we analyze the recent distribution of tropical seagrass beds, their temporal changes, causes of decline and conservation status in Southeast Asia (plus southe

rapidly.2 This makes it unlikely that increased local blood flow will result in increased tissue concentrations in the steady state, though there is the possibility of an effect where frequent intravenous injections result in rapidly rising plasma levels. In 1987 our patient had three intravenous injections of phenytoin, two of 200 mg and one of 300 mg, but not before or since. While acute ischaemia can cause hyperperfusion,' this usually persists longer than the four days between the original S

Abstract Japanese kelps are important because they perform a valuable ecosystem function of coastal kelp forest formation, and they are important food resources. The present study aimed to predict future shifts in major kelp species in northern Japan under different climate change scenarios. From a database of cold temperate kelp species in Japan, we extracted 1,958 data points to estimate the distribution of 11 kelp species that inhabit the waters around northern Japan. Distributions of the pas

The features or mechanisms of dyshidrosis have not been sufficiently clarified. Neither has the difference between hyperhidrosis and hypohidrosis. To clarify the features and mechanisms of dyshidrosis (hyperhidrosis and hypohidrosis) in syringomyelia, the clinical features focusing on hidrosis of 30 patients with syringomyelia and Chiari malformation located from a syringomyelia database were prospectively analysed. The patients were classified into three groups: eight patients (26.7%) had segme

In this study, we have developed a practical mouse model for evaluating in vivo the antiviral activity of compounds against respiratory syncytial virus (RSV) infection. BALB/c mice are not particularly susceptible to RSV infection; however, infection rates were improved by pretreatment with the immunosuppressive agent cyclophosphamide (CYP). When mice were inoculated intranasally with RSV A2 strain, the pulmonary RSV titres of CYP-pretreated 10-week-old mice were higher than those of untreated 1

This study evaluated the inhibitory effects of 2,4,6-trihydroxy,3-nitro-benzamide (THNB) derivatives against hepatitis C virus (HCV). protease and other human serine proteases. The inhibitory efficacy was tested with a reversed-phase HPLC assay system using a NS3-NS4A fusion protein as the HCV protease and a synthetic peptide substrate that mimics the NS5A-5B junction. Twelve THNB derivatives showed more than 50% inhibition at 100 μg mL-1. The most potent derivative was RD3-4082, with 50% inhibi

Hepatitis C virus (HCV) NS3-NS4A protease is an attractive target for anti-HCV agents because of its important role in replication. An optimized fluorescence resonance energy transfer (FRET) substrate for NS3-NS4A protease, based on the sequence of the NS5A-5B cleavage site, was designed and synthesized. High-throughput screening of in-house compound libraries was performed using a FRET substrate FS10 (MOCAcDKIVPC-SMSYK-Dnp) and MBP-NS3-NS4A fusion protein. Several hit compounds were found, incl

RD3-0028, a benzodithiin compound, has antiviral activity against respiratory syncytial virus (RSV) in cell culture. We used a mouse model of RSV infection to determine the in vivo effect of RD3-0028. Cyclophosphamide (CYP)-treated, immunosuppressed mice were inoculated intranasally. The lungs of the mice were removed on day 4. The virus titers of the lungs of RD3-0028-treated mice were compared to the virus titers of the lungs of virus-inoculated, untreated control mice. In an effort to increas