Kyoto University · Biochemistry, Genetics and Molecular Biology
Professor Kiyoshi Yasukawa's research lab specializes in molecular biology and biotechnology, with a focus on cytokine receptor biology and reverse transcriptase enzymology. The lab investigates the structure and function of interleukin-6 receptor (IL-6R), particularly soluble IL-6R, using recombinant protein expression and immunoassay techniques. It also explores the biochemical properties of reverse transcriptases from retroviruses, emphasizing their thermal stability and activity modulation by nucleic acid templates. These studies contribute to the development of sensitive detection systems and tools for molecular diagnostics and gene expression analysis.
Figures are computed from collected data and may differ slightly.
An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sIL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1 cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recombinant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant sIL-6R augmented the sensi
Reverse transcriptases (RTs) from avian myeloblastosis virus (AMV) and Moloney murine leukaemia virus (MMLV) have been most extensively used as a tool for conversion of RNA to DNA. In this study, we compared the thermal stabilities of AMV RT and MMLV RT by observing their irreversible thermal inactivation. The temperatures reducing initial activity by 50% in 10-min incubation, T(50), of AMV RT were 47 degrees C without the template-primer (T/P), poly(rA)-p(dT)(12-18), and 52 degrees C with the T
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