The University of Osaka · Biochemistry, Genetics and Molecular Biology
Professor Kohsuke Honda's research lab specializes in synthetic and systems biology, focusing on the design and engineering of artificial metabolic pathways for sustainable chemical production. The lab pioneers *in vitro* metabolic engineering by reconstituting multi-enzyme cascades in a test tube, enabling efficient and modular biosynthesis of industrial chemicals without the constraints of living cells. A key innovation involves the use of artificial operons to co-express multiple thermophilic enzymes with controlled expression levels, enhancing pathway efficiency and stability. The lab also develops advanced biocatalytic systems that integrate enzymes from diverse biological sources to create novel, high-yielding metabolic routes.
Figures are computed from collected data and may differ slightly.
In vitro reconstitution of an artificial metabolic pathway is an emerging approach for the biocatalytic production of industrial chemicals. However, several enzymes have to be separately prepared (and purified) for the construction of an in vitro metabolic pathway, thereby limiting the practical applicability of this approach. In this study, genes encoding the nine thermophilic enzymes involved in a non-ATP-forming chimeric glycolytic pathway were assembled in an artificial operon and co-express
Bio-based chemical production has drawn attention regarding the realization of a sustainable society. <i>In vitro</i> metabolic engineering is one of the methods used for the bio-based production of value-added chemicals. This method involves the reconstitution of natural or artificial metabolic pathways by assembling purified/semi-purified enzymes <i>in vitro</i>. Enzymes from distinct sources can be combined to construct desired reaction cascades with fewer biological constraints in one vessel
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