Pohang University of Science and Technology · Biochemistry, Genetics and Molecular Biology
Professor Kyong-Taek Kim's research lab specializes in cellular signaling and calcium dynamics, with a focus on the physiological mechanisms underlying hormone secretion in neuroendocrine cells. The lab investigates the distinct roles of intracellular and extracellular calcium sources in regulating exocytosis, particularly in bovine adrenal medullary cells, using advanced techniques such as fura-2 ratiometric imaging and amperometric detection. Their work reveals fundamental differences in the functional outcomes of calcium mobilization from internal stores versus extracellular influx, highlighting the specialized regulatory roles of each calcium pool. The lab also explores the impact of pharmacological agents, such as dimethyl isobutylamine, on calcium signaling and secretory processes.
Figures are computed from collected data and may differ slightly.
Bovine adrenal medullary cells, cultured on quartz plates, were superfused with buffer to which pulses of stimulant were added. Cytosolic Ca2+ was measured by the fura-2 fluorescence method and the simultaneously released catecholamine was measured electrochemically. When stimulant concentrations were adjusted to given equivalent elevations of cytosolic Ca2+, secretion depended entirely on whether Ca2+ came from internal stores or from the extracellular medium. Calcium from internal stores did n
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