Waseda University · Environmental Science
Professor Satoshi Tsuneda's research lab focuses on microbial ecology and environmental biotechnology, with a strong emphasis on understanding the roles of extracellular polymeric substances (EPS) in microbial community dynamics, particularly in wastewater treatment systems. The lab investigates microbial interactions in biofilms and granules, exploring how EPS components influence cell adhesion, biofilm formation, and nitrification/denitrification processes. A key research direction involves deciphering the host-microbe interactions in the gut, especially the modulation of intestinal barrier function by probiotic bacteria and their potential therapeutic applications in inflammatory bowel disease. The lab employs an integrative approach combining molecular microbiology, metabolomics, and mathematical modeling to address environmental and medical challenges.
Figures are computed from collected data and may differ slightly.
The influence of extracellular polymeric substances (EPS) on bacterial cell adhesion onto solid surfaces was investigated using 27 heterotrophic bacterial strains isolated from a wastewater treatment reactor. Cell adhesion onto glass beads was carried out by the packed-bed method and the results were discussed in terms of the amount of each EPS component produced and cell surface characteristics such as zeta potential and hydrophobicity. Protein and polysaccharides accounted for 75-89% of the EP
Epithelial barrier dysfunction has been implicated as one of the major contributors to the pathogenesis of inflammatory bowel disease. The increase in intestinal permeability allows the translocation of luminal antigens across the intestinal epithelium, leading to the exacerbation of colitis. Thus, therapies targeted at specifically restoring tight junction barrier function are thought to have great potential as an alternative or supplement to immunology-based therapies. In this study, we screen
The imbalance of gut microbiota is known to be associated with inflammatory bowel disease, but it remains unknown whether dysbiosis is a cause or consequence of chronic gut inflammation. In order to investigate the effects of gut inflammation on microbiota and metabolome, the sequential changes in gut microbiota and metabolites from the onset of colitis to the recovery in dextran sulfate sodium-induced colitic mice were characterized by using meta 16S rRNA sequencing and proton nuclear magnetic
This study evaluates the community structure in nitrifying granules (average diameter of 1600 mum) produced in an aerobic reactor fed with ammonia as the sole energy source by a multivalent approach combining molecular techniques, microelectrode measurements and mathematical modelling. Fluorescence in situ hybridization revealed that ammonia-oxidizing bacteria dominated within the first 200 mum below the granule surface, nitrite-oxidizing bacteria a deeper layer between 200 and 300 mum, while he
Open papers in the app to read, cite, and organize with AI.
