Yoshiaki Uchida

Immunotherapy with daily intradermal injections of OK-432, penicillin- and heat-treated lyophilized powder of Su-strain of streptococcus pyogens A3, for over a period of four weeks resulted in quantitative and qualitative effectiveness on impaired cell-mediated immunity even in many patients with far advanced cancer of the stomach or lung. In vitro lymphocyte studies following immunotherapy with OK-432 demonstrated restoration of circulating lymphocyte counts to more than 1,500/microliters, a le

Omnidirectional laser action in W/O/W emulsion with CLC shells is demonstrated. The emulsion, in which the helical axis of the CLC phase is normal to the surface, is successfully fabricated by using a microfluidic device. The whole emulsion system with dispersed core-shell droplets with a CLC shell can serve as a flexible omnidirectional resonator for dye lasers.

Twelve patients with carcinomatous pleural effusions were treated with single intrapleural (i.pl.) administration of OK432 on day 0 and the effects of i.pl. OK432 on natural killer (NK) cell activity were followed on day 7. Two patients showed no clinical evidence of therapeutic benefit from i.pl. OK432. In the other 10 patients, pleural effusions and/or tumor cells in the effusions had decreased or disappeared by day 7. NK cell activity was markedly low or absent in pleural effusions of untreat

Natural killer (NK) cell activity was examined in breast cancer patients before and after surgery, and the cells involved in the postoperative depression of cytotoxicity were characterized. NK activity against K562 target cells determined in 4-hour 51Cr release assay of blood lymphocytes from preoperative patients was comparable to that of normal donors. After surgery the patients showed a decrease in NK activity but not in number of large granular lymphocytes in the effector cell populations. W

Human lymphocytes and their subpopulations from the peripheral blood and pleural effusions of cancer patients were tested for cytotoxicity against fresh tumor cells isolated from carcinomatous pleural effusions of the same patients. Fresh tumor cells were relatively resistant to lysis by autologous unseparated lymphocytes in a 4 h Cr-release assay. Positive reactions were recorded in 10 of 38 blood samples and 10 of 37 effusion specimens. Purification of large granular lymphocytes (LGL) by disco

Lymphocytes from peripheral blood (PBL) and from pleural effusions (PEL) of cancer patients were tested for cytotoxicity against tumor cells freshly isolated from carcinomatous pleural effusion of the same patient. Significant lysis of autologous tumor cells was recorded for 4 of 28 PBL samples and for 5 of 28 PEL cases when investigated in a 4-hour 51Cr release assay. In vitro treatment of lymphocytes for 20 hours with the streptococcal preparation OK432 resulted in an induction or augmentation

Fifty patients with primary localized lung cancer were tested at the time of surgery for the ability of their lymphocytes to kill autologous, freshly isolated tumor cells, and the assay was evaluated for prognostic significance. Peripheral blood lymphocytes of 27 patients (54%) demonstrated significant autologous tumor-killing activity in 6-hour 51Cr-release assays. Twenty-three of the 27 patients with autologous tumor-killing activity remained tumor free and survived more than 5 years after cur

An anisotropic and inhomogeneous magnetic interaction (the average spin-spin interaction constant (-)J > 0) was observed in the various liquid crystalline (LC) phases of racemic and nonracemic all-organic radical LC compounds 1a and 1b. We discussed how the LC superstructures induced the magnetic interaction to operate in the LC phases in terms of spin-spin dipole and exchange interactions by means of VT-EPR spectroscopy. The magnitude of the magnetic interaction depended on the type of LC phase

Bone marrow natural killer (NK) cell activity was studied in patients with multiple myeloma. Bone marrow mononuclear cells from myeloma patients expressed considerable levels of cytotoxicity against K562 in a 4 h 51Cr-release assay, which was comparable to that of blood lymphocytes. In contrast, NK-cell activity was markedly low or absent in bone marrow of normal donors and control patients. Fractionation of bone marrow cells from myeloma patients on linear bovine serum albumin gradients enriche

Here we show the first observation of an unusual intermolecular magnetic interaction in the liquid-crystalline (LC) state of an all-organic radical compound at 73 °C on water; the magnetic interaction actually allows the LC droplet on water to be attracted by a weak permanent magnet, whereas the crystalline phase did not respond to the magnet.