Yujiro Hirose

The arginine deiminase (ADI) pathway has been found in many kinds of bacteria and functions to supplement energy production and provide protection against acid stress. The Streptococcus pyogenes ADI pathway is upregulated upon exposure to various environmental stresses, including glucose starvation. However, there are several unclear points about the advantages to the organism for upregulating arginine catabolism. We show that the ADI pathway contributes to bacterial viability and pathogenesis u

<i>Streptococcus pyogenes</i> is a major cause of necrotizing fasciitis, a life-threatening subcutaneous soft-tissue infection. At the host infection site, the local environment and interactions between the host and bacteria have effects on bacterial gene expression profiles, while the gene expression pattern of <i>S. pyogenes</i> related to this disease remains unknown. In this study, we used a mouse model of necrotizing fasciitis and performed RNA-sequencing (RNA-seq) analysis of <i>S. pyogene

Dental pulp stem cells (DPSCs) are reported as sources of mesenchymal stem cells (MSCs). MSCs are used as cell therapy options for various diseases. The present study examined the healing effects of DPSC injection on damaged bladder tissue in a chemically induced cystitis rat model. Cystitis was induced by hydrochloride injection into the bladder of female F344/NSlc rats. On the following day, DPSCs suspended in phosphate-buffered saline (PBS) were injected into the bladder and maintained for 1

<i>Streptococcus pyogenes</i> can cause a wide variety of acute infections throughout the body of its human host. An underlying transcriptional regulatory network (TRN) is responsible for altering the physiological state of the bacterium to adapt to each unique host environment. Consequently, an in-depth understanding of the comprehensive dynamics of the <i>S. pyogenes</i> TRN could inform new therapeutic strategies. Here, we compiled 116 existing high-quality RNA sequencing data sets of invasiv

Streptococcus pneumoniae is a major pathogen that causes pneumonia, sepsis, and meningitis. The candidate combox site 4 (ccs4) gene has been reported to be a pneumococcal competence-induced gene. Such genes are involved in development of S. pneumoniae competence and virulence, though the functions of ccs4 remain unknown. In the present study, the role of Ccs4 in the pathogenesis of pneumococcal meningitis was examined. We initially constructed a ccs4 deletion mutant and complement strains, then

Invasive infection caused by <i>Streptococcus pyogenes emm</i>89 strains has been increasing in several countries linked to a recently emergent clade of <i>emm</i>89 strains, designated clade 3. In Japan, the features of <i>emm</i>89 <i>S. pyogenes</i> strains, such as clade classification, remains unknown. In this study, we collected <i>emm</i>89 strains isolated from both streptococcal toxic shock syndrome (STSS) (89 STSS isolates) and noninvasive infections (72 non-STSS isolates) in Japan fro

<i>Streptococcus pyogenes</i> is responsible for a range of diseases in humans contributing significantly to morbidity and mortality. Among more than 200 serotypes of <i>S. pyogenes</i>, serotype M1 strains hold the greatest clinical relevance due to their high prevalence in severe human infections. To enhance our understanding of pathogenesis and discovery of potential therapeutic approaches, we have developed the first genome-scale metabolic model (GEM) for a serotype M1 <i>S. pyogenes</i> str

The crystal microstructure of copper interconnects in ultra large scale integrations (ULSIs), depends on factors such as the trench line width and patterns. Therefore, inner crystal characterisation of the desired trench lines composed of copper is required. Electron backscatter diffraction pattern (EBSP) inspection has become a powerful technique for analysing the crystal characterisation of interconnects. However, EBSP inspection is sensitive to surface conditions such as contamination and str

We propose an effective method for carrier injection into the high-energy Γ ground state in a narrow quantum well (QW) in asymmetric double QW systems using Γ–X–Γ electron transfer and simultaneous hole tunneling. Although the high-energy state is type-II band-aligned for the electrons, our method enables an efficient injection of both electrons and holes into the same narrow QW, and it thus demonstrates relatively strong light emission from the higher energy state.

The development of a safe and efficient method for isolation of mesenchymal stem cell (MSC) subsets is essential for optimal stem cell therapy. The potential utility of granulocyte-colony stimulating factor (G-CSF) induced mobilization method has been demonstrated in isolation of MSC subsets from dental pulp. The mobilized dental pulp stem cells (MDPSCs) were enriched for MSC subsets with higher regenerative potential compared with colony-derived dental pulp stem cells (DPSCs). This investigatio

Abstract Streptococcus pyogenes can cause a wide variety of acute infections throughout the body of its human host. The underlying transcriptional regulatory network (TRN) is responsible for altering the physiological state of the bacterium to adapt to each host environment. Consequently, an in-depth understanding the comprehensive dynamics of its TRN could inform new therapeutic strategies. Here, we compiled 116 existing high-quality RNA-seq data sets of S. pyogenes serotype M1, and estimated t