[论文解读] Alterations in cell surface area and deformability of individual human red blood cells in stored blood

HyunJoo Park, Misook Ji+5 more|arXiv (Cornell University)|Jun 17, 2015

Digital Holography and Microscopy参考文献 51被引用 5

一句话总结

本研究利用定量相位成像技术分析储存过程中单个人类红细胞（RBCs）的变化，发现CPDA-1保存液可将RBC形态和变形性维持至6周，而未保存的RBC在2周内迅速转化为球形红细胞，其表面积和变形性显著降低。

ABSTRACT

The functionality and viability of stored human red blood cells (RBCs) is an important clinical issue in transfusion. To systematically investigate changes in stored whole blood, the hematological properties of individual RBCs were quantified in blood samples stored for various periods with and without a preservation solution called CPDA-1. With 3-D quantitative phase imaging techniques, the optical measurements of the 3-D refractive index (RI) distributions and membrane fluctuations were done at the individual cell level. From the optical measurements, the morphological (volume, surface area and sphericity), biochemical (hemoglobin content and concentration), and mechanical parameters (dynamic membrane fluctuation) were simultaneously quantified to investigate the functionalities and their progressive alterations in stored RBCs. Our results show that the stored RBCs without CPDA-1 had a dramatic morphological transformation from discocytes to spherocytes within 2 weeks which was accompanied with significant decreases in cell deformability and cell surface area, and increases in sphericity. However, the stored RBCs with CPDA-1 maintained their morphology and deformability for up to 6 weeks.

研究动机与目标

• 研究血液储存过程中单个人类红细胞的形态和力学特性随时间的渐进性变化。
• 评估CPDA-1保存液对RBC功能随时间的影响。
• 在单细胞水平上量化表面积、变形性、体积和球形度的变化。
• 将折射率和膜波动的光学测量结果与RBC功能衰退相关联。
• 确定无保存液条件下RBC退化的时序过程。

提出的方法

• 采用三维定量相位成像测量单个RBC的折射率（RI）分布。
• 从光学相位数据量化体积、表面积和球形度等形态学参数。
• 通过测量动态膜波动作为RBC变形性的指标。
• 同时从RI图中提取血红蛋白含量和浓度等生化参数。
• 在多个时间点（最长6周）比较添加和未添加CPDA-1的RBC。
• 利用光学测量推断RBC的力学和生化特性，无需标记或固定。

实验结果

研究问题

• RQ1 在无CPDA-1的条件下，单个RBC的细胞表面积和变形性在储存过程中如何变化？
• RQ2 CPDA-1在6周储存期内对RBC形态和机械功能的保护作用有多大？
• RQ3 储存RBC中从双凹盘状向球形红细胞的形态转化的时序过程是怎样的？
• RQ4 折射率和膜波动的变化如何与储存RBC的功能衰退相关联？
• RQ5 储存RBC中球形度、表面积与变形性之间存在何种关系？

主要发现

• 未添加CPDA-1储存的RBC在2周内经历了从双凹盘状向球形红细胞的显著形态转变。
• 未保存的RBC在储存2周后表面积显著减少，与变形性丧失相关。
• 未保存的RBC在第2周时变形性明显下降，表现为膜波动振幅减小。
• 未保存的RBC球形度显著增加，证实其向球形形态转变。
• 添加CPDA-1的RBC在长达6周内维持正常的双凹盘状形态、稳定的表面积和较高的变形性。
• CPDA-1保存的RBC中折射率和血红蛋白浓度保持相对稳定，表明其生化完整性随时间保持良好。