[论文解读] CPTCs Drive Somatic-Visceral Communication via the Wnt Axis in Somatic Mechanotherapy: A Single-Cell Deep Learning Study

Haixiang Huang, Zhenwei Zhang+11 more|arXiv (Cornell University)|Feb 5, 2026

Cellular Mechanics and Interactions被引用 0

一句话总结

该研究使用可解释的单细胞深度学习框架表明 CD34+PDGFRα+ 纤毛细胞（CPTCs）在体细胞力治疗中充当机械感应器，激活一个由 Wnt 驱动的再生信号轴以修复结肠上皮。

ABSTRACT

Somatic mechanical stimulation (e.g., acupuncture) exerts systemic immunomodulatory effects, yet the cellular bridge translating peripheral physical force into visceral repair remains elusive. Here, employing a custom interpretable deep learning framework (CARSS) on single-cell RNA sequencing data, we identify CD34$^{+}$PDGFR$α$$^{+}$ telocytes (CPTCs) as the primary mechanosensors in both fascia and colon during bacterial colitis. We show that somatic mechanotherapy triggers an AP-1/Hsp70-dependent transcriptional program in fascial CPTCs, inducing systemic Wnt elevation, which elicits a "transcriptional resonance" in colonic CPTCs, reprogramming their communication network from an inflammatory amplifier to a Wnt-driven regenerative hub. Mechanistically, this axis activates epithelial $β$-catenin/Myc signaling, suppressing apoptosis and restoring barrier integrity independent of immune cells. Our findings define a CPTC-Driven Mechano-Resonance Axis, where CPTCs serve as synchronized relay stations that convert local mechanical cues into systemic regenerative microenvironments.

研究动机与目标

• Identify cellular bridges translating local mechanical cues into systemic visceral responses during somatic mechanotherapy.
• Discover CPTCs as primary mechanosensors in fascia and colon during bacterial colitis.
• Characterize transcriptional programs and signaling axes linking somatic stimulation to colonic regeneration.

提出的方法

• Develop a custom interpretable deep learning framework (CARSS) to analyze single-cell RNA sequencing data.
• Identify CPTCs as the main mechanosensors in fascia and colon under colitis.
• Characterize AP-1/Hsp70-dependent transcriptional programs and consequent systemic Wnt signaling.
• Map intercellular communication networks and their reprogramming from inflammatory to regenerative states.

实验结果

研究问题

• RQ1 What cells mediate somatic mechanical cues to elicit visceral repair?
• RQ2 Do CPTCs act as mechanosensors in both fascia and colon during colitis?
• RQ3 How does somatic mechanotherapy alter transcriptional programs and intercellular networks to promote regeneration via Wnt signaling?

主要发现

• Somatic mechanotherapy engages CPTCs as primary mechanosensors in fascia and colon during colitis.
• An AP-1/Hsp70-dependent transcriptional program in fascial CPTCs triggers systemic Wnt elevation.
• Colonic CPTCs experience a transcriptional resonance, shifting from inflammatory signaling to a Wnt-driven regenerative hub.
• Wnt axis activates epithelial β-catenin/Myc signaling and suppresses epithelial apoptosis, aiding barrier restoration independent of immune cells.