Kwang Soo Kim

Given the therapeutic potential offered by embryonic stem (ES) cells, it is critical to optimize stable gene delivery and expression at different developmental stages of ES cell differentiation. Here, we systematically analyzed lentiviral vectors containing the following promoters: the human elongation factor 1alpha (EF1alpha) promoter, the human cytomegalovirus (CMV) immediate early region enhancer-promoter, the composite CAG promoter (consisting of the CMV immediate early enhancer and the chic

The ability of empirical models to enhance accuracy of site-specific estimates of leaf wetness duration (LWD) was assessed for 15 sites in Iowa, Nebraska, and Illinois during May to September of 1997, 1998, and 1999. Enhanced estimation of LWD was obtained by applying a 0.3-m height correction to SkyBit wind-speed estimates for input to the classification and regression tree/stepwise linear discriminant (CART/SLD) model (CART/SLD/Wind model), compared to either a proprietary model (SkyBit wetnes

Transplantation of mouse embryonic stem (mES) cells can restore function in Parkinson disease models, but can generate teratomas. Purification of dopamine neurons derived from embryonic stem cells by fluorescence-activated cell sorting (FACS) could provide a functional cell population for transplantation while eliminating the risk of teratoma formation. Here we used the tyrosine hydroxylase (TH) promoter to drive enhanced green fluorescent protein (eGFP) expression in mES cells. First, we evalua

Journal Article Rapid communication: linkage and physical mapping of the porcine melanocortin-4 receptor (MC4R) gene Get access K. S. Kim, K. S. Kim 4Department of Animal Science, Iowa State University, Ames 50011, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar N. J. Larsen, N. J. Larsen 4Department of Animal Science, Iowa State University, Ames 50011, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar M. F. Rothschild M. F. R

ABSTRACT Few biologically based models to assess the risk of soybean rust have been developed because of difficulty in estimating variables related to infection rate of the disease. A fuzzy logic system, however, can estimate apparent infection rate by combining meteorological variables and biological criteria pertinent to soybean rust severity. In this study, a fuzzy logic apparent infection rate (FLAIR) model was developed to simulate severity of soybean rust and validated using data from fiel

Empirical models based on classification and regression tree analysis (CART model) or fuzzy logic (FL model) were used to forecast leaf wetness duration (LWD) 24 h into the future, using site-specific weather data estimates as inputs. Forecasted LWD and air temperature then were used as inputs to simulate performance of the Melcast and TOM-CAST disease-warning systems. Overall, the CART and FL models underpredicted LWD with a mean error (ME) of 2.3 and 3.9 h day -1 , respectively. The CFL model,

We propose a weighted ensemble approach using a surrogate variable. As a case study, the degree of agreement (DOA) statistics for potential evapotranspiration (PET) was determined to compare the ordinary arithmetic mean ensemble (OAME) method and the surrogate weighted mean ensemble (SWME) method for three domains. Solar radiation was used as the surrogate variable to determine the weight values for the ensemble members. Singular vector decomposition with truncation values was used to select fiv

The genus Jeffersonia, which contains only two species, has a trans-Atlantic disjunct distribution. The aims of this study were to determine the requirements for breaking dormancy and germination of J. dubia seeds and to compare its dormancy characteristics with those of the congener in eastern North America. Ripe seeds of J. dubia contain an underdeveloped embryo and were permeable to water. In nature, seeds were dispersed in May, while embryos began to grow in September, and were fully elongat