Ji-Hoon Ryu
Korea University · 生化学・遺伝学・分子生物学
研究室紹介
Professor Ji-Hoon Ryu's research lab focuses on food safety and microbial control, particularly targeting pathogenic bacteria such as Escherichia coli O157:H7. The lab investigates the role of bacterial extracellular structures—like curli and exopolysaccharides—in biofilm formation, environmental persistence, and resistance to chemical sanitizers such as chlorine and chlorine dioxide. A key research direction involves developing effective, non-thermal decontamination methods for fresh produce and seeds that preserve viability while ensuring pathogen inactivation. The lab also explores the interplay between microbial surface structures and their interactions with food contact surfaces, such as stainless steel, to improve sanitation strategies in the food industry.
Research Overview
Research Output Trend
Figures are computed from collected data and may differ slightly.
Selected Papers
15The resistance of Escherichia coli O157:H7 strains ATCC 43895-, 43895-EPS (an exopolysaccharide [EPS]-overproducing mutant), and ATCC 43895+ (a curli-producing mutant) to chlorine, a sanitizer commonly used in the food industry, was studied. Planktonic cells of strains 43895-EPS and/or ATCC 43895+ grown under conditions supporting EPS and curli production, respectively, showed the highest resistance to chlorine, indicating that EPS and curli afford protection. Planktonic cells (ca. 9 log(10) CFU
AIMS: The aim of this study was to determine the role of curli in attachment and biofilm formation by Escherichia coli O157:H7 on stainless steel. METHODS AND RESULTS: Three curli-deficient strains (43895-, 43894- and E0018-) and three curli over-producing strains (43895+, 43894+ and E0018+) of E. coli O157:H7 were studied. Stainless steel coupons (SSC) were immersed in cell suspensions of each strain for 24 h at 4 degrees C. The number of cells attached to SSC was determined. To determine the a