Ki-Hong Choi
Korea University · 化学
研究室紹介
Professor Ki-Hong Choi's research lab specializes in the design and synthesis of functional molecules for biological sensing and probing, with a focus on fluorescent probes for redox biology and anion recognition. The lab develops ratiometric and turn-on fluorescent probes—particularly based on coumarin and cyanoacrylamide scaffolds—to monitor dynamic cellular processes such as glutathione levels in stem cells and anion binding in real time. A key research direction involves creating macrocyclic receptors with tailored cavities for selective anion recognition, enabling sensitive detection through photoinduced processes. The lab also investigates molecular interactions in ribosomal translation, using photo-crosslinking techniques to map the path of nascent peptides in the ribosome.
Research Overview
Research Output Trend
Figures are computed from collected data and may differ slightly.
Selected Papers
15A macrocycle containing an aminocoumarin fluorophore showed two different fluorescence emission bands on binding with certain anions. Proton transfer from the fluorophore excited state occurred depending on the basicity of the bound anion (see scheme, A−: anion) and this new pathway makes more-sensitive anion sensing possible.
The core functions of stem cells (SCs) are critically regulated by their cellular redox status. Glutathione is the most abundant non-protein thiol functioning as an antioxidant and a redox regulator. However, an investigation into the relationship between glutathione-mediated redox capacity and SC activities is hindered by lack of probe. Here, we demonstrate that cyanoacrylamide-based coumarin derivatives are ratiometric probes suitable for the real-time monitoring of glutathione levels in livin
The cyclic triamide of 3'-amino-3-biphenylcarboxlic acid is readily synthesized in a stepwise manner and represents a novel class of anion receptors with a large central cavity. This macrocycle binds more strongly to tetrahedral anions than spherical or planar anions in organic solvents. The binding stoichiometries for anions with symmetrical charge distribution depend on the solvent polarity, while tetrahedral p-tosylate binds to the macrocycle with 1:1 stoichiometry in all solvents studied. Th
As part of a programme to investigate the path of the nascent peptide through the large ribosomal subunit, peptides of different lengths (up to 30 amino acids), corresponding to the signal peptide sequence and N-terminal region of the Escherichia coli ompA protein, were synthesized in situ on E.coli ribosomes. The peptides each carried a diazirine moiety attached to their N-terminus which, after peptide synthesis, was photoactivated so as to induce cross-links to the 23S rRNA. The results showed
2,2-Diphenyl-[1,3]dioxolane-4,5-dicarboxylic acid (DPD) and 2,2-dinaphthalen-2-yl-[1,3]dioxolane-4,5-dicarboxylic acid (DND) have been synthesized starting from dimethyl tartrate. DPD and DND amides of alpha-chiral primary amines showed significantly different (1)H chemical shift values depending on the stereochemistry of the derivatizing agent. On the basis of this chemical shift difference, the absolute configuration of amine substrates could be assigned. DND amides showed significantly larger
On the basis of our modeling of pillared covalent organic frameworks (PCOFs) with pyridine molecules inserted between the COF-1 layers, we propose that the surface area and free volume of storage materials should be balanced to increase the gravimetric and volumetric hydrogen uptake capacities. Density functional theory and grand canonical Monte Carlo simulations show that these PCOFs have significantly improved gravimetric and volumetric hydrogen storage capacities of 8.8–10.0 wt % and 58.7–61.
Ein Makrocyclus mit einem Aminocumarin-Fluorophor zeigt nach Bindung bestimmter Anionen zwei Fluoreszenzemissionsbanden. Ein Protonentransfer vom angeregten Zustand des Fluorophors erfolgt in Abhängigkeit von der Basizität des gebundenen Anions (siehe Schema, A− = Anion), was einen empfindlicheren Anionennachweis ermöglicht. Supporting information for this article is available on the WWW under http://www.angewandte.com or from the author. Please note: The publisher is not responsible for the con