Keio University · 의학
Akiyoshi Hirayama 교수의 연구실은 주로 대사체학(메타볼로믹스)을 기반으로 한 임상 대사체 분석에 초점을 맞추고 있습니다. 특히 캐피러리 전기영동-질량분석법(CE-MS)을 활용해 생체체액 및 종양 조직 내의 전하를 띤 대사물질을 정밀하게 정량 분석하며, 암 등 질병에서의 대사 변화를 규명하는 데 기여하고 있습니다. 연구는 임상 샘플의 전처리 조건 최적화와 고감도 분석 기술 개발을 통해 정량적 신뢰도를 높이는 데에도 초점을 맞추고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Most cancer cells predominantly produce energy by glycolysis rather than oxidative phosphorylation via the tricarboxylic acid (TCA) cycle, even in the presence of an adequate oxygen supply (Warburg effect). However, little has been reported regarding the direct measurements of global metabolites in clinical tumor tissues. Here, we applied capillary electrophoresis time-of-flight mass spectrometry, which enables comprehensive and quantitative analysis of charged metabolites, to simultaneously mea
The development of high-throughput metabolite measurement technologies has enabled the use of metabolomics for epidemiologic studies by profiling metabolite concentrations in large cohorts of human blood samples. Standard protocols are necessary to obtain unbiased profiles through multiple runs over long periods of time and to allow reliable statistical analyses. This study assessed the effects of sampling procedures and storage conditions on the stability of metabolomic profiles in plasma and s
Sheath-flow capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a new tool for comprehensive analysis of charged metabolites. However, it needs to be more sensitive. Here, we report a sheathless capillary electrophoresis-electrospray ionization-mass spectrometry method for cationic metabolome analysis. This system used a high-sensitivity porous sprayer interface and 10% (v/v) acetic acid as the background electrolyte (BGE). Under optimized conditions, 53 cationic metabolites, incl
