Tokyo Institute of Technology · 생화학·유전·분자생물학
마루야마 아츠시 교수의 연구실은 생물학적 고분자, 특히 DNA와 다핵산의 상호작용을 중심으로 한 생고분자 공학 및 나노재료 개발을 주요 연구 분야로 삼고 있습니다. 특히, DNA 삼중나선 안정화, 다핵산 기반 나노입자 설계, 그리고 유전자 조절을 위한 고분자 기반 벡터 개발에 초점을 맞추고 있으며, 생체 내 유전자 발현 조절 메커니즘의 실시간 탐지 기술 개발도 진행 중입니다. 이는 유전자 치료 및 정밀의료에 응용 가능한 기초 기술을 확립하는 데 기여하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Phylogenetic positions of psychrophilic bacteria isolated from the Japan Trench were determined by sequencing analysis of PCR-amplified bacterial small subunit (16S) rRNA genes. Between surface and deep-sea psychrophiles, distinct positions clearly differed within the gamma-Proteobacteria. In phylogenetic analysis using neighbour-joining, maximum-parsimony and maximum-likelihood, strains from surface seawater were inferred to be located in the Halomonas aquamarina-meridiana clade within the fami
Biodegradable nanoparticles, which contain the sites for both polynucleotide adsorption and targeting ligand on their surfaces, were prepared as a novel carrier for genetic materials. The nanoparticles were obtained from poly(D,L-lactic acid) and poly(L-lysine)-graft-polysaccharide copolymers by using either a solvent evaporation method or a diafiltration method. The size of the particles prepared by the diafiltration method was controlled by varying the initial concentration of the graft copoly
DNA triplex formation has been studied as a potential strategy for regulation of gene expression. The triplex is, however, unstable under physiological conditions, so that an effective stabilizer for the triplex formation is needed. Here is shown a novel strategy to stabilize the triplex based on the molecular design of a comb-type polycation. Linear polycations, such as poly(L-lysine) and poly(L-arginine), thermally stabilize DNA duplexes (and triplexes). The complexes between DNA and the polyc
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnantioselective permeation of α-amino acid isomers through poly(amino acid)-derived membranesAtsushi Maruyama, Noriyuki Adachi, Takehisa Takatsuki, Masanori Torii, Kohei Sanui, and Naoya OgataCite this: Macromolecules 1990, 23, 10, 2748–2752Publication Date (Print):May 1, 1990Publication History Published online1 May 2002Published inissue 1 May 1990https://pubs.acs.org/doi/10.1021/ma00212a027https://doi.org/10.1021/ma00212a027research-articleACS Publi
The polyionic interaction between DNA and polycations grafted with hydrophilic dextran side chains was evaluated. The comb-type copolymers, poly(L-lysine)-graft-dextran, were successfully prepared by employing a reductive amination reaction between epsilon-amino groups of poly(L-lysine) (PLL) and the reductive ends of dextran (Dex). A coupling efficacy on the order of 70% was obtained regardless of intrinsic philicities of the solvents used, either aqueous buffer or DMSO. The resulting graft cop
Using a luciferase reporter assay, we previously demonstrated that a Z-DNA-forming sequence of alternating thymine-guanine repeats in the human heme oxygenase-1 gene (HO-1) promoter is involved in nuclear factor erythroid-derived 2 (NF-E2)-related factor 2 (Nrf2)-mediated HO-1 promoter activation. However, the actual Z-DNA formation in this native genomic locus has not been experimentally demonstrated. To detect Z-DNA formation in vivo, we generated a construct containing the Z-DNA-binding domai
