Hokkaido University · 지구·행성과학
아마구치 아츠시 교수의 연구실은 유전자 코드 확장 기술을 핵심으로 하여, 비표준 아미노산를 단백질에 정밀하게 통합하는 고도화된 분자 생물학 기법을 개발하고 있습니다. 특히 피롤루실-tRNA 리아제(PylRS) 시스템을 활용한 단백질 접합 및 기능화 기술을 응용해, 항체 및 단백질 공학 분야에서의 응용 가능성을 탐색하고 있습니다. 또한, 해양 생물의 생태학적 분포와 생애 주기 연구를 통해 해양 생물 다양성과 생태계 기능을 이해하는 데에도 기여하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Z-Lysine (ZLys) is a lysine derivative with a benzyloxycarbonyl group linked to the ε-nitrogen. It has been genetically encoded with the UAG stop codon, using the pair of an engineered variant of pyrrolysyl-tRNA synthetase (PylRS) and tRNA(Pyl). In the present study, we designed a novel Z-lysine derivative (AmAzZLys), which is doubly functionalized with amino and azido substituents at the meta positions of the benzyl moiety, and demonstrated its applicability for creating protein conjugates. AmA
Genetic code expansion has largely relied on two types of the tRNA-aminoacyl-tRNA synthetase pairs. One involves pyrrolysyl-tRNA synthetase (PylRS), which is used to incorporate various lysine derivatives into proteins. The widely used PylRS from Methanosarcinaceae comprises two distinct domains while the bacterial molecules consist of two separate polypeptides. The recently identified PylRS from <i>Candidatus</i> Methanomethylophilus alvus (CMaPylRS) is a single-domain, one-polypeptide enzyme t
Diel and seasonal vertical distribution, life cycle and body allometry of Pleuromamma scutullata and Heterorhabdus tanneri were investigated in the Oyashio region during September 1996 through October 1997. Monthly samples were collected with 0.1 mm mesh closing nets towed through five discrete depths between the surface and 2000 m. Copepodite stages 1 through 6 of P.scutullata and 3 through 6 of H.tanneri were collected effectively with the nets. Day-night samplings during December 1996, April
Population structure and life cycle of Pseudocalanus minutus and P. newmani in Toyama Bay, southern Japan Sea, were investigated based on seasonal samples obtained by vertical hauls (0-500 m depth) of twin-type Norpac nets (0.33-mm and 0.10-mm mesh) over one full year from February 1990 through January 1991. Closing PCP nets (0.06-mm mesh) were also towed to evaluate vertical distribution patterns in September 1990, November 1991 and February 1997. P. minutus was present throughout the year. The
Indeep water, most of the calanoid copepod community consisted of cosmopolitan species, while an endemiccommunity was observed in the subarctic region. Because the food of deep-sea calanoid copepods originates fromthe surface layer, sufficient and excess flux in the eutrophic subarctic region may be responsible for maintaining the endemic species in the region.
As habitats of pelagic copepods, epipelagic oceanic environments are characterized by greater food availability but higher risks of predation. Both food supply and predation risk rapidly drop with increasing depth. We studied day/night vertical distribution patterns of copepodid stages of 6 epipelagic (Neocalanus cristatus, N. flemingeri, N. plumchrus, Eucalanus bungii, Metridia pacifica, and M. okhotensis), 2 mesopelagic (Gaetanus simplex and Pleuromamma scutullata), and 6 bathypelagic copepods
