Dal‐Hee Min
서울대학교 · Biochemistry, Genetics and Molecular Biology
Dal-Hee Min 교수의 연구실은 나노소재 기반의 약물 전달 및 유전자 치료제 개발에 초점을 맞추고 있습니다. 특히 메조다공성 실리카 나노입자를 활용한 고효율 siRNA 전달 시스템과, 단백질 키나제 활성 분석을 위한 레이블 프리 질량분석 기반의 고속 스크리닝 기술을 개발하고 있습니다. 또한 RNA 기반 치료제의 안정성과 체내 전달 문제를 해결하기 위한 나노구조재료를 활용한 다기능 유전자 조절 시스템도 연구하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Among various nanoparticles, mesoporous silica nanoparticles (MSNs) have attracted extensive attention for developing efficient drug-delivery systems, mostly due to their high porosity and biocompatibility. However, due to the small pore size, generally below 5 nm in diameter, potential drugs that are loaded into the pore have been limited to small molecules. Herein, a small interfering RNA (siRNA) delivery strategy based on MSNs possessing pores with an average diameter of 23 nm is presented. T
Weighing in: Multiple kinases can be assayed simultaneously by using a method that combines peptide chips and MALDI-TOF mass spectrometry (MS). The method uses self-assembled monolayers that present a set of peptides that are each selective for a kinase. The kinase phosphorylates the peptide, changing its mass. MS analysis of the surface resolves each peptide and gives the activity of each kinase. Herein we describe a strategy that combines peptide chips with matrix-assisted laser desorption/ion
Abstract Recently, there have been significant advances in the field of RNA‐based therapeutics harnessing exogenous RNAs including small interfering RNA (siRNA), microRNA (miRNA) mimics, and in vitro transcribed (IVT) mRNAs. RNA‐based therapeutics are one of the most attractive classes of drugs for treating a variety of diseases. In principle, RNA‐based therapeutics offer numerous advantages compared to the conventional small‐molecule‐based therapies. However, there are several drawbacks to over
The multifunctional DNAzyme (Dz) delivery system is developed based on nano-sized graphene oxide (nGO) for simultaneous detection and knockdown of the target gene. The Dz/nGO complex system allowed convenient monitoring of HCV mRNA in living cells and silencing of the HCV gene expression by Dz-mediated catalytic cleavage concurrently.
This paper reports an enzyme activity assay that combines the assets of both homogeneous and solid-phase formats. In this method, enzyme reactions are carried out in solution using substrates that are tagged with an immobilization reagent that allows the substrates to be selectively immobilized to self-assembled monolayers (SAMs), for direct analysis by matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). As a model enzyme reaction, this work examined th
