The University of Osaka · 공학
이 교수의 연구실은 레이블 프리(optical biosensor) 기반의 생물학적 분자 상호작용을 실시간으로 감지할 수 있는 고감도 센서 기술 개발에 초점을 맞추고 있습니다. 주로 국소 표면 플라즈몬 공 resonance(LSPR) 원리를 활용한 나노구조 기반 센서를 설계하여, 암 관련 유전자, 우유 알레르기 유발 단백질 등 임상 및 환경 모니터링에 활용 가능한 진단 기술을 개발하고 있습니다. 또한, 마이크로플루이딕 기반 RT-PCR 칩을 통합한 빠른 바이러스 진단 플랫폼 개발을 통해 감염병의 신속한 진단과 확산 방지를 위한 기술 혁신을 추구하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
The development of label-free optical biosensors for DNA and other biomolecules has the potential to impact life sciences as well as screening in medical and environmental applications. In this report, we developed a localized surface plasmon resonance (LSPR) based label-free optical biosensor based on a gold-capped nanoparticle layer substrate immobilized with peptide nucleic acids (PNAs). PNA probe was designed to recognize the target DNA related to tumor necrosis factor. The nanoparticle laye
AbstractIn this research, a localized surface plasmon resonance (LSPR) immunosensor based on gold-capped nanoparticle substrate for detecting casein, one of the most potent allergens in milk, was developed. The fabrication of the gold-capped nanoparticle substrate involved a surface-modified silica nanoparticle layer (core) on the slide glass substrate between bottom and top gold layers (shell). The absorbance peak of the gold-capped nanoparticle substrate was observed at ∼520 nm. In addition, t
In recent years, label-free biosensors not requiring external modifications have been receiving intense attention. A label-free optical biosensor, which retains many of the desirable features of conventional surface plasmon resonance (SPR) reflectometry, namely, the ability to monitor the kinetics of biomolecular interactions in real-time without a label has been developed with several important advantages: the biosensor device is easy to fabricate, and simple to implement, requiring only an UV–
Rapid and definitive diagnosis is critical to the prevention of the spread of endemic human pathogenic viruses. Detection of variant specific genes by reverse transcription polymerase chain reaction (RT-PCR) has become a routine diagnostic test for accurate subtyping of RNA viruses, such as influenza. In this paper, we demonstrate the use of a continuous-flow polydimethylsiloxane (PDMS) microfluidic RT-PCR chip and disposable electrical printed (DEP) chips for rapid amplification and sensing of
