경희대학교 · 농업·생명과학
이 교수의 연구실은 식품 및 동물에서 유래한 유산균, 병원성 세균, 항생제 내성 유전자 등을 중심으로 식품안전과 미생물 정확한 동정을 위한 분자생물학적 및 유전체 기반 기술을 개발하고 있습니다. 특히 Lactobacillus, Lacticaseibacillus, Staphylococcus, Enterococcus 등 주요 식품미생물의 정밀 동정과 probiotic 기능 평가, 항생제 내성 유전자 모니터링에 초점을 맞추고 있습니다. MALDI-TOF MS와 유전자 기반 분석을 융합한 빠르고 정확한 식품미생물 검출 기술 개발이 핵심 연구 과제입니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
The method developed in this study is able to rapidly and accurately distinguish different species of Lactobacillus, and can be used to monitor specific Lactobacillus species in foods such as probiotics and dairy products.
<i>Lacticaseibacillus casei</i>, <i>Lacticaseibacillus chiayiensis</i>, and <i>Lacticaseibacillus zeae</i> are very closely related <i>Lacticaseibacillus</i> species. <i>L. casei</i> has long been proposed as a probiotic, whereas studies on functional characterization for <i>L. chiayiensis</i> and <i>L. zeae</i> are some compared to <i>L</i>. <i>casei</i>. In this study, <i>L. casei</i> FBL6, <i>L. chiayiensis</i> FBL7, and <i>L. zeae</i> FBL8 were isolated from raw milk, and their probiotic pro
<i>Staphylococcus</i> species have a ubiquitous habitat in a wide range of foods, thus the ability to identify staphylococci at the species level is critical in the food industry. In this study, we performed rapid identification of <i>Staphylococcus</i> species using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS was evaluated for the identification of <i>Staphylococcus</i> reference strains (<i>n</i> = 19) and isolates (<i>n</i> = 96) f
Coagulase-positive <i>Staphylococcus aureus</i> is a foodborne pathogen considered one of the causes of food-related disease outbreaks. Like <i>S. aureus</i>, <i>Staphylococcus capitis</i>, <i>Staphylococcus caprae</i>, and <i>S. epidermidis</i> are opportunistic pathogens causing clinical infections and food contamination. The objective of our study was to develop a rapid, accurate, and monitoring technique to detect four <i>Staphylococcus</i> species in food. Four novel molecular targets (GntR
The use of phenicol antibiotics in animals has increased. In recent years, it has been reported that the transferable gene mediates phenicol-oxazolidinone resistance. This study analyzed the prevalence and characteristics of phenicol-oxazolidinone resistance genes in <i>Enterococcus faecalis</i> and <i>Enterococcus faecium</i> isolated from food-producing animals and meat in Korea in 2018. Furthermore, for the first time, we reported the genome sequence of <i>E. faecalis</i> strain, which posses
Because of their beneficial effects, some enterococci species are used in probiotics, whereas some species are considered opportunistic pathogens implicated in human infections. High 16S rRNA sequence similarities among Enterococcus can impede their accurate classification. In this study, using pan-genome analysis, we developed an accurate method for the identification and quantification of six major Enterococcus species in different foods. Bioinformatics analysis revealed that some Enterococcus
Kimchi, a traditional Korean fermented vegetable, has received considerable attention for its health-promoting effects. This study analyzes the cultivable microbial community in kimchi fermented at different temperatures using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to comprehensively understand the factors affecting the quality of kimchi. Of the 5204 strains isolated from kimchi, aligned with the in-house database, 4467 (85.8%) were correctly
