Tohoku University · 생화학·유전·분자생물학
Fumi Nagatsugi 교수의 연구실은 타겟 유전자에 고도로 선택적으로 작용하는 화학적 접근법을 통해 유전자 발현을 조절하는 데 초점을 맞추고 있습니다. 특히, 이완형 DNA 구조를 이용한 삼중서열 형성 올리고뉴클레오티드(TFO)와 반응성 기능기를 갖춘 뉴클레오시드 유도체를 결합하여 사이토신이나 티미딘과의 선택적 교차결합을 실현합니다. 이는 유전자 편집, 사이토신 기반의 정밀 변형, 그리고 miRNA 기반 유전자 조절 기법 개발로 이어지는 핵심 기반 기술입니다. 연구는 주로 유전자 조절, 약물 타겟팅, 분자 유전자 기법의 개발에 응용됩니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTHighly Efficient and Selective Cross-Linking to Cytidine Based on a New Strategy for Auto-Activation within a DuplexFumi Nagatsugi, Takeshi Kawasaki, Daisaku Usui, Minoru Maeda, and Shigeki SasakiView Author Information Graduate School of Pharmaceutical Sciences Kyushu University, 3-1-1 Maidashi Higashi-ku, Fukuoka 812-8582, Japan Cite this: J. Am. Chem. Soc. 1999, 121, 28, 6753–6754Publication Date (Web):July 3, 1999Publication History Received5
Interstrand cross-linking (ICL) forming oligodeoxynucleotides (ODNs) have been expected to ensure the inhibition of gene expression. In this communication, we report a highly efficient and selective ICL reaction to thymine using a 4-amino-2-vinyl-6-oxopyrimidine derivative.
The specific recognition of homopurine-homo pyrimidine regions in duplex DNA by triplex-forming oligonucleotides (TFOs) provides an attractive strategy for genetic manipulation. Alkylation of nucleobases with functionalized TFOs would have the potential for site-directed mutagenesis. Recently, we demonstrated that a TFO bearing 2-amino-6-vinylpurine derivative, 1, achieves triplex-mediated reaction with high selectivity toward the cytosine of the G-C target site. In this report, we have investig
Synthetic oligonucleotides (ONs) are valuable tools that interfere with gene expression by specifically binding to target genes in a sequence-specific manner. Reactive ONs containing cross-linking agents are expected to induce efficient inhibition because they bind covalently to target genes. In recent years, researchers have reported several cross-linking reactions that target DNA induced by external stimuli. This short review highlights recently developed novel cross-linking reactions, focusin
Masking the miRNA binding site: Crosslink-forming oligonucleotide (CFO) was used for target gene-specific inhibition of microRNA (miRNA) functions. This method can interfere with specific miRNA-mRNA interactions by recognizing sequences unique to the 3'-UTR that are inherent in each mRNA.
Selective chemical reactions with DNA, such as its labelling, are very useful in many applications. In this paper, we discuss a new strategy for the selective alkylation of DNA using an oligonucleotide containing an abasic site and alkylating probes. We designed three probes consisting of 2-AVP as a reactive moiety and three kinds of binding moiety with high affinity to duplex DNA. Among these probes, Hoechst-AVP probe exhibited high selectivity and efficient reactivity to thymine bases at the s
Guanine (G)-rich sequences can form non-canonical four stranded structures known as G-quadruplexes (G4s). The G4 structure has been found in biologically significant regions of the genomic DNA, and many G4 ligands with a high specificity and affinity have been reported. In this review, we focus on the functional G4 ligands, which not only bind to the G4 structure but also have some function, especially with inducing G4 folding, chemical reactions, and alkylation by proximity. G-quadruplexes (G4s
