포항공과대학교 · 생화학·유전·분자생물학
Gyoo Yeol Jung 교수의 연구실은 대사 공학과 합성 생물학을 기반으로 대사 경로의 정밀 제어와 효율적 대사 유량 조절을 목표로 합니다. 특히 효소의 알로스테릭 조절 메커니즘을 이해하고 변형함으로써 대사 경로의 기능적 최적화를 도모하며, 단일 세포 수준에서의 대사물질 모니터링 및 고고도 스크리닝 기술을 접목한 혁신적 생물공학 플랫폼을 개발하고 있습니다. 이는 친환경적이고 경제적인 생물학적 합성 공정의 실현을 위한 핵심 기반 기술입니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Control of enzyme allosteric regulation is required to drive metabolic flux toward desired levels. Although the three-dimensional (3D) structures of many enzyme-ligand complexes are available, it is still difficult to rationally engineer an allosterically regulatable enzyme without decreasing its catalytic activity. Here, we describe an effective strategy to deregulate the allosteric inhibition of enzymes based on the molecular evolution and physicochemical characteristics of allosteric ligand-b
One of the great advantages of microbial fermentation is the capacity to convert various carbon compounds into value-added chemicals. In this regard, there have been many efforts to engineer microorganisms to facilitate utilization of abundant carbon sources. Recently, the potential of acetate as a feedstock has been discovered; efforts have been made to produce various biochemicals from acetate based on understanding of its metabolism. In this review, we discuss the potential sources of acetate
Pathway optimization is difficult to achieve owing to complex, nonlinear, and largely unknown interactions of enzymes, regulators, and metabolites. We report a pathway reconstruction using RNA display–derived messenger RNA–enzyme fusion molecules. These chimeras are immobilized by hybridization of their messenger RNA end with homologous capture DNA spotted on a substrate surface. Enzymes thus immobilized retain activity proportional to the amount of capture DNA, allowing modulation of the relati
