早稲田大学 · Biochemistry, Genetics and Molecular Biology
하루카 테이야마 교수의 연구실은 주로 미생물 및 단세포 생물의 대사 공학과 유전자 기반 생물자원 개발을 핵심으로 합니다. 특히 에우글레나나 청색세균을 활용한 항산화물질(비타민 C, E, 베타카로틴) 및 오메가-3 지방산(EPA)의 효율적 생산 기술 개발에 중점을 두고 있으며, 단일세포 유전자 분석 기술을 통해 유전적 다양성과 생물합성 경로를 정밀하게 규명하고 있습니다. 이는 환경 친화적인 바이오소재 및 기능성 식품 원료 개발에 기여합니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Euglena gracilis Z is one of the few microorganisms which simultaneously produces antioxidant vitamins such as beta-carotene and vitamins C and E. Photoheterotrophically cultured E. gracilis Z produced larger levels of biomass but with a lower content of antioxidant vitamins than photoautotrophically grown cultures. For efficient production of these vitamins, a two-step culture was performed. Cells were grown photoheterotrophically and then transferred to photoautotrophic conditions. When E. gra
The eicosapentaenoic acid (EPA) synthesis gene cluster isolated from a marine bacterium, Shewanella putrefaciens strain SCRC-2738, was cloned and expressed in the marine cyanobacterium Synechococcus sp. A broad-host-range cosmid vector, pJRD215 (10.2 kb, Smr Kmr), was used to clone a 38 kb insert, pEPA, containing the EPA synthesis gene cluster, creating plasmid pJRDEPA (approx. 48 kb). This plasmid was transferred to the cyanobacterial host at a frequency of 2.2 x 10(-7). Cyanobacterial transco
Whole genome amplification (WGA) is essential for obtaining genome sequences from single bacterial cells because the quantity of template DNA contained in a single cell is very low. Multiple displacement amplification (MDA), using Phi29 DNA polymerase and random primers, is the most widely used method for single-cell WGA. However, single-cell MDA usually results in uneven genome coverage because of amplification bias, background amplification of contaminating DNA, and formation of chimeras by li
Restriction analysis detected two types of bigeye tuna (α and β); the α type was in the majority in the Atlantic but nearly absent in the Indo‐Pacific. The β type shared a larger number of restriction sites with other species than the conspecific β type, but bigeye‐specific nucleotide substitutions with a novel diagnostic restriction profile were found. Although the nucleotide sequence difference between Atlantic and Pacific sub‐species of the northern bluefin tuna was nearly the largest among s
