Hee Taek Kim
이화여자대학교 생화학·유전학·분자생물학과 · 생화학·유전·분자생물학
Hee Taek Kim 교수의 연구실은 생물학적 전환 기반의 지속 가능한 화학공업을 목표로 하며, 폴리에스터 플라스틱의 생물학적 업사이클링과 유용한 생분해성 화학물질의 대량 생산을 핵심 연구 방향으로 삼고 있습니다. 특히 PET 플라스틱을 테레프탈산으로 분해하고 이를 카테콜 등 고부가가치 화합물로 전환하는 케미오-생물학적 융합 기술을 개발하고 있으며, 라이신에서 유도되는 카데바린 등의 다이아민을 이용한 생분해성 폴리아미드 제조 기술도 선도하고 있습니다. 연구는 대량 생산이 가능한 미생물을 기반으로 한 대사공학과 효소 공학을 융합하여 실용화 가능한 생물정제 공정을 목표로 합니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Poly(ethylene terephthalate) (PET), composed of terephthalic acid (TPA) and ethylene glycol (EG), is the most commonly produced polyester. Unrecycled PET waste causes serious environmental problems. To increase the PET recycling rate, the upcycling of PET into products that are higher value than PET is desired. In this study, the feasibility of biological valorization of PET for its upcycling was experimentally evaluated. Among the two monomers obtained from the chemical hydrolysis of PET, TPA w
Fermentative production of cadaverine from renewable resources may support a sustainable biorefinery process to produce carbon-neutral nylons such as biopolyamide 510 (PA510). Cost-competitive production of cadaverine is a key factor in the successful commercialization of PA510. In this study, an integrated biological and chemical process involving cadaverine biosynthesis, purification, and its polymerization with sebacic acid was developed to produce bio-PA510. To stably express ldcC from Esche
Chemo-biological upcycling of poly(ethylene terephthalate) (PET) developed in this study includes the following key steps: chemo-enzymatic PET depolymerization, biotransformation of terephthalic acid (TPA) into catechol, and its application as a coating agent. Monomeric units were first produced through PET glycolysis into bis(2-hydroxyethyl) terephthalate (BHET), mono(2-hydroxyethyl) terephthalate (MHET), and PET oligomers, and enzymatic hydrolysis of these glycolyzed products using Bacillus su
Lysine decarboxylases (LDCs) from Escherichia coli, Lactobacillus saerimneri, Streptomyces coelicolor, Selemonas ruminantium, Hafnia alvei, and Vibrio vulnificus were examined for their ability to enhance the fermentative production of cadaverine in Corynebacterium glutamcium. Among these LDCs, the plasmid-based expression of the H. alvei LDC gene (ldcCHa) under strong promoters (PH30, PH36) produced high concentrations of cadaverine (11.4–11.5 g/L), which is similar to 12.5 g/L of cadaverine pr
We report metabolic engineering of Corynebacterium glutamicum (C. glutamicum) for high-level production of 5-hydroxyvaleric acid (5-HV), an important C5 platform chemical covering a wide range of industrial applications, using glucose as a sole carbon source. To derive 5-HV, an artificial 5-HV biosynthesis pathway, composed of the first three reaction steps of an l-lysine catabolic pathway via 5-aminovaleramide along with a subsequent intracellular reduction step, was constructed: l-lysine was c
Cadaverine is a C5 diamine monomer used for the production of bio-based polyamide 510. Cadaverine is produced by the decarboxylation of l-lysine using a lysine decarboxylase (LDC). In this study, we developed recombinant <i>Escherichia coli</i> strains for the expression of LDC from <i>Hafnia alvei</i>. The resulting recombinant XBHaLDC strain was used as a whole cell biocatalyst for the high-level bioconversion of l-lysine into cadaverine without the supplementation of isopropyl β-d-1-thiogalac
An all-inclusive bio-chemical route from the fermentation process to downstream process for C5 plasticizer synthesis was developed using fermentation-derived glutaric acid produced by metabolically engineered Corynebacterium glutamicum .
