京都大学 · Biochemistry, Genetics and Molecular Biology
Hiroshi Sugiyama 교수의 연구실은 핵산의 고차구조, 특히 G-구름(G-quadruplex)과 이중나선 DNA의 상호작용 메커니즘을 분자 오비탈 계산과 실험 기법을 융합하여 연구합니다. 특히 DNA의 광분해 특이성, 특히 5'-GG-3' 염기서열에서의 고유한 반응성 기원을 전산화학적 접근으로 규명하며, 암 치료용 분자 타겟팅 약물 개발에 기여하고자 합니다. 연구는 분자간 상호작용, 구조 안정성, 그리고 생물학적 기능 간의 연관성을 중심으로 전개됩니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Ab initio molecular orbital calculations of stacked DNA bases were performed at the 3-21G(*) and 6-31G* levels to elucidate the origin of the 5‘-GG-3‘ sequence specificity for the photocleavage of DNA in the presence of electron-accepting photosensitizers. Ionization potentials (IP) were estimated as Koopman's theorem values for 16 sets of two stacked nucleobases and seven sets of stacked nucleobase pair systems in a B-form geometry. It was found that the GG/CC system is the lowest among the 10
A G-quadruplex (G4) is a well-known nucleic acid secondary structure comprising guanine-rich sequences, and has profound implications for various pharmacological and biological events, including cancers. Therefore, ligands interacting with G4s have attracted great attention as potential anticancer therapies or in molecular probe applications. To date, a large variety of DNA/RNA G4 ligands have been developed by a number of laboratories. As protein-targeting drugs face similar situations, G-quadr
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHighly sequence-selective photoreaction of 5-bromouracil-containing deoxyhexanucleotidesHiroshi Sugiyama, Yasushi Tsutsumi, and Isao SaitoCite this: J. Am. Chem. Soc. 1990, 112, 18, 6720–6721Publication Date (Print):August 1, 1990Publication History Published online1 May 2002Published inissue 1 August 1990https://pubs.acs.org/doi/10.1021/ja00174a046https://doi.org/10.1021/ja00174a046research-articleACS PublicationsRequest reuse permissionsArticle Views
Intramolecular folding in three tandem guanine repeats of human telomeric DNA has been investigated using optical-tweezers, MD simulation and circular dichroism. A mechanically and thermodynamically stable species in this sequence shows a structure consistent with a triplex conformation. A similar species has also been observed to coexist with a G-quadruplex in a DNA sequence with four tandem guanine repeats.
