Kyushu University · 의학
이 교수의 연구실은 간 기능 재생과 인공간 기반 치료를 목표로 하며, 다세포 구형체(spheroids)를 활용한 고기능성 간세포 배양 기술과 고체 기질 기반 하이브리드 인공간 시스템 개발에 중점을 두고 있습니다. 특히 폴리우레탄 폼 기반 셀룰러 모듈을 이용한 간세포 생체외 유지 및 간부전 모델에서의 생존율 향상에 성공하였으며, 탈세포화된 간세포외기질(L-ECM)을 활용한 세포 기반 재생의학 기법도 개발하고 있습니다. 이는 향후 임상적 적용이 가능한 인공간 지원 시스템 및 조직공학적 응용에 기여할 잠재력을 지닙니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Spherical multicellular aggregates (spheroids) of adult rat hepatocytes were spontaneously formed in the pores of polyurethane foam (PUF) as a culture substratum. Highly differentiated functions of hepatocytes, that is, albumin secretion, urea synthesis, and drug metabolism, were maintained in the spheroid culture using PUF. These spheroids were partly attached and immobilized in the pores of PUF. Then, we designed a PUF/spheroids packed-bed module as a hybrid artificial liver and developed an a
Monkey kidney cells (Vero), human embryonic kidney cells (293), human liver cells (PLC/PRF/5), and primary rat, dog, and porcine hepatocytes formed spherical multicellular aggregates (spheroids) in the pores of polyurethane foam which was used as a cell culture substratum. These spheroids of various cell types express high cell activity for a long period. A practical hybrid artificial live support system composed of a multi-capillary polyurethane foam packed-bed type cell culture module includin
The decellularization of organs has attracted attention as a new functional methodology for regenerative medicine based on tissue engineering. In previous work we developed an L-ECM (Extracellular Matrix) as a substrate-solubilized decellularized liver and demonstrated its effectiveness as a substrate for culturing and transplantation. Importantly, the physical properties of the substrate constitute important factors that control cell behavior. In this study, we aimed to quantify the physical pr
The extracellular matrix (ECM) in a liver-specific extracellular matrix (L-ECM) scaffold facilitates hepatocyte viability and maintains hepatocyte functions <i>in vitro</i>. However, whether an intact composition of ECM is required for an efficient ECM-based substrate design remains to be clarified. In this study, two L-ECM hydrogels, namely L-ECM I and L-ECM II, were prepared by pepsin solubilization at 4 °C and 25 °C, respectively. The solubility at 4 °C was 50% whereas that at 25 °C was 95%,
The hep-col scaffold can localize several kinds of growth factors as well as stabilize bFGF under physiological temperature and is a promising potent scaffold for regenerative medicine.
