京都大学 · Biochemistry, Genetics and Molecular Biology
카즈나리 아키요시 교수의 연구실은 수소화된 다당류를 기반으로 한 나노구조체, 특히 콜레스테롤 기반 페룰란(cholesterol-bearing pullulan)을 이용한 자가조직화 나노입자 및 수성 젤 나노소재의 설계와 응용을 핵심으로 합니다. 이들은 단백질 리폴딩을 촉진하는 분자촉매 유사 기능을 가진 나노겔을 개발하여 단백질 안정화 및 복구에 응용하며, 뼈 재생을 위한 성장인자 전달 시스템도 개발하고 있습니다. 특히, 사이클로덱스트린과의 상호작용를 통한 단백질 포획 및 방출 메커니즘은 생물의학적 응용에서 높은 잠재력을 지닙니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-aggregates of hydrophobized polysaccharides in water. Formation and characteristics of nanoparticlesKazunari Akiyoshi, Shigeru Deguchi, Nobuhiro Moriguchi, Shigehiko Yamaguchi, and Junzo SunamotoCite this: Macromolecules 1993, 26, 12, 3062–3068Publication Date (Print):June 1, 1993Publication History Published online1 May 2002Published inissue 1 June 1993https://pubs.acs.org/doi/10.1021/ma00064a011https://doi.org/10.1021/ma00064a011research-article
Various cholesterol-bearing pullulans (CHPs) with different molecular weights of the parent pullulan and degrees of substitution (DS) of the cholesteryl moiety were synthesized. The structural characteristics of CHPs in water were studied by static (SLS) and dynamic light scattering (DLS) and the fluorescence probe method. Irrespective of the molecular weight of the parent pullulan and the DS, all of CHPs provided unimodal and monodisperse self-aggregates in water. The size of the self-aggregate
Molecular chaperone-like activity for protein refolding was investigated using nanogels of self-assembly of cholesterol-bearing pullulan. Nanogels effectively prevented protein aggregation (i.e. carbonic anhydrase and citrate synthase) during protein refolding from GdmCl denaturation. Enzyme activity recovered in high yields upon dissociation of the gel structure in which the proteins were trapped, by the addition of cyclodextrins. The nanogels assisted protein refolding in a manner similar to t
We have been studying the formation of hydrogel nanoparticles by the self-aggregation of hydrophobized polysaccharide and the effective complexation between these nanoparticles as a host and various globular soluble proteins as a guest. This paper describes a new finding that refolding of the heat-denatured enzyme effectively occurs with the nanoparticles and beta-cyclodextrin according to a mechanism similar to that of a molecular chaperone. In particular, the irreversible aggregation of carbon
Thermoresponsive hydrogel nanoparticles were prepared by self-assembly of two different hydrophobically modified polymers, namely a cholesterol-bearing pullulan (CHP) and a copolymer of N-isopropylacrylamide (NIPAM) and N-[4-(1-pyrenyl)butyl]-N-n-octadecylacrylamide] (PNIPAM-C18Py). The interactions between CHP and PNIPAM-C18Py were investigated by fluorescence spectroscopy, dynamic light scattering, and size exclusion chromatography. After ultrasonication of a mixture of CHP and PNIPAM-C18Py (5
Bone regeneration for the defects in revision surgery of joint replacement is an increasingly important issue. To repair bone defects, bone cell activation by growth factors using synthetic resorbable scaffold is a useful and safe option. We examine the efficiency of nanogel-crosslinking hydrogel as a novel synthetic scaffold for BMP to stimulate osteoblasts and to induce bone formation. Cholesterol-bearing pullulan nanogel-crosslinking hydrogel (CHPA/Hydrogel) was used to deliver BMP. The CHPA
