Tohoku University · 환경과학
Kengo Kubota 교수의 연구실은 환경 미생물학 분야에서 활동하며, 특히 환경 샘플 내 미생물의 정밀 탐지와 분류를 목표로 하고 있습니다. 주로 FISH(형광 현미경 기반 유전자 탐지 기술)와 CARD-FISH 기반의 신호 증폭 기술을 활용해, 환경 내 미생물의 다양성과 기능을 정량적으로 분석하고 있습니다. 또한 락스드-핵산(LNA)을 활용한 고감도 프로브 개발을 통해 기존 기술의 한계를 극복하고자 연구를 진행하고 있습니다. 최근에는 혐기성 슬러지 담체에서의 진핵생물 군집 구조 분석을 통해 미생물 생태계의 이해를 심화하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Fluorescence in situ hybridization (FISH) has become a standard technique in environmental microbiology. More than 20 years have passed since this technique was first described, and it is currently used for the detection of ribosomal RNA, messenger RNA, and functional genes encoded on chromosomes. This review focuses on the advancement and applications of FISH combined with catalyzed reporter deposition (CARD, also known as tyramide signal amplification or TSA), in the detection of environmental
Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. In this study, we demonstrate remarkable improvement in in situ hybridization efficiency by applying locked-nucleic-acid (LNA)-incorporated oligodeoxynucleotide probes (LNA/DNA probes) without compromising specificity. Fluorescently
ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTTwo New Quassinoids, Ailantinols A and B, and Related Compounds from Ailanthus altissimaKengo Kubota, Narihiko Fukamiya, Tomomi Hamada, Masayoshi Okano, Kiyoshi Tagahara, and Kuo-Hsiung LeeView Author Information Department of Interdisciplinary Studies of Natural Environment, Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739, Faculty of Pharmaceutical Sciences, Kobe Pharmaceutical University, Kobe 658, Japan, and Natural
Eukaryotic communities in aerobic wastewater treatment processes are well characterized, but little is known about them in anaerobic processes. In this study, abundance, diversity and morphology of eukaryotes in anaerobic sludge digesters were investigated by quantitative real-time PCR (qPCR), 18S rRNA gene clone library construction and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Samples were taken from four different anaerobic sludge digesters in Japan. Result
