Kyoto University · 공학
케미 교수의 연구실은 주로 혈액줄기세포 및 만성질환 관련 세포의 정밀한 생물학적 분석을 위한 마이크로유체 기반의 혁신적 플랫폼을 개발하고 있습니다. 특히 인간 복합줄기세포(hPSC)의 다배열성 유지와 실시간 동적 모니터링을 가능하게 하는 3차원 마이크로유체 환경 설계, 그리고 나노입자 기반 약물 전달 시스템의 생체 내 행동을 규명하는 데 초점을 맞추고 있습니다. 또한, 생물학적 환경에서의 세포 반응 제어 및 유전자 수준의 진화적 적응 분석을 통해 의약품 개발과 재생의료의 기반을 마련하고자 합니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
We have successfully designed and fabricated an integrated microfluidic platform, the hESC-microChip, which is capable of reproducible and quantitative culture and analysis of individual hESC colonies in a semi-automated fashion. In this device, a serpentine microchannel allows pre-screening of dissociated hESC clusters, and six individually addressable cell culture chambers enable parallel hESC culture, as well as multiparameter analyses in sequence. In order to quantitatively monitor hESC prol
Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL(-1) of bFGF on 20 microg mL(-1) of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering
Abstract Aus drei Bausteinen entstehen auf der Grundlage eines Cyclodextrin‐Adamantan‐Erkennungssystems supramolekulare Nanopartikel (SNPs) variabler Größe (30–450 nm). Die Bioverteilung und Lymphknotendrainage der SNPs in Mäusen wurde mit Positronenemissionstomographie untersucht. Die Größen der SNPs beeinflussen ihr In‐vivo‐Verhalten (siehe Bild). magnified image
Abstract We have grown thick AlN epilayers on SiC substrates by a new solution growth technique using Cu solvents under atmospheric pressure nitrogen. By using growth apparatus based on CZ growth system with inductive heating, we have grown AlN single crystalline layers of which thickness were more than 200 μm on (4H,6H)‐SiC substrates at relatively low growth temperatures such as 1600 °C‐1800 °C. Inch‐size self standing AlN crystals were also prepared by removing the SiC substrate. TEM observat
Human pluripotent stem cells hold great promise for applications in drug discovery and regenerative medicine. Microfluidic technology is a promising approach for creating artificial microenvironments; however, although a proper 3D microenvironment is required to achieve robust control of cellular phenotypes, most current microfluidic devices provide only 2D cell culture and do not allow tuning of physical and chemical environmental cues simultaneously. Here, the authors report a 3D cellular micr
In mammalian evolutionary history, Cetacea (whales, dolphins and porpoises) achieved astonishing success by adapting to an aquatic environment. One unique characteristic of cetaceans, contributing to this adaptive success, is efficient lipid utilization. Here, we report a comparative genetic analysis of five aquatic and five terrestrial Cetartiodactyla species using 144 genes associated with lipid metabolism. Mutation ratio (d<sub>N</sub> /d<sub>S</sub> ), amino acid substitution in functional d
Abstract Synthesis of single crystalline AlN has long been the subjects of intensive studies since it has exceptional properties suitable for the substrate materials for optoelectronic and electronic devices. The solution growth technique has some advantages over the sublimation growth technique. Its growth temperature is generally much lower than that of the sublimation growth. The obtained crystal is believed to show superior crystallinity since it is grown under nearly equilibrium condition.
Microfabrication materials such as polydimethylsiloxane (PDMS) and photoresists (PRs) permit the creation of nano/microstructured substrates that enable the direct control of the cellular functions and phenotypes of human pluripotent stem cells (hPSCs). The multiple analyses of cellular phenotypes and gene expression demonstrate that microfabrication materials preferentially direct hPSCs towards differentiation.
Current anticancer treatments have many limitations to achieving high efficacy. Hence, novel strategies that broaden therapeutic prospects must urgently be developed. Ferroptosis is an iron-dependent form of non-apoptotic programmed cell death that is induced by cellular antioxidative system inhibition. Photodynamic therapy (PDT) uses photosensitizers to generate reactive oxygen species and aggravate oxidative stress in tumor cells. Combining ferroptosis with PDT cooperatively regulates intracel
