Waseda University · 농업·생명과학
코스케 카타오카 교수의 연구실은 미토콘드리아 생물학과 단백질 품질 관리 시스템에 중점을 두고 있으며, 특히 라이브스톡 유전자학과 신경생물학적 모델로 활용되는 메뚜기류 및 메뚜기(개미핥이)의 미토콘드리아 게놈 분석을 통해 진화적 유전체학적 관계를 규명하고 있습니다. 또한, 미토콘드리아 내 단백질 분해 효소인 Lon 프로테아제와 관련된 질병 관련 단백질인 Cereblon의 기능적 역할, 특히 산화 스트레스에 대한 세포 보호 기전을 탐구하고 있습니다. 이와 더불어, 세포 부착 및 기계적 자극에 대한 세포 반응을 연구하기 위해 생체막재료(PDMS)의 표면 기능화 기술을 응용한 세포 생물학적 연구도 수행하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
DATA REPORT article Front. Genet., 08 May 2020Sec. Livestock Genomics Volume 11 - 2020 | https://doi.org/10.3389/fgene.2020.00470
Species of infraorder Gryllidea, or crickets, are useful invertebrate models for studying developmental biology and neuroscience. They have also attracted attention as alternative protein sources for human food and animal feed. Mitochondrial genomic information on related invertebrates, such as katydids, and locusts, has recently become available in attempt to clarify the controversial classification schemes, although robust phylogenetic relationships with emphasis on crickets remain elusive. He
The online version contains supplementary material available at 10.1007/s12551-021-00924-4.
Lon protease plays a major role in the protein quality control system in mammalian cell mitochondria. It is present in the mitochondrial matrix, and degrades oxidized and misfolded proteins, thereby protecting the cell from various extracellular stresses, including oxidative stress. The intellectual disability-associated and thalidomide-binding protein cereblon (CRBN) contains a large, highly conserved Lon domain. However, whether CRBN has Lon protease-like function remains unknown. Here, we det
Surface modification of polydimethylsiloxane (PDMS) with an extracellular matrix (ECM) is useful for enhancing stable cell attachment. However, few studies have investigated the correlation between the stability of deposited ECM and cell behavior on the PDMS surfaces in external stretched cell culture systems. Herein, covalent collagen type I (Col)-immobilized PDMS surfaces were fabricated using 3-aminopropyl-trimethoxysilane, glutaraldehyde, and Col molecules. The immobilized collagen molecules
Abstract The two-spotted cricket, Gryllus bimaculatus , is a key hemimetabolous model organism for developmental biology, neuroscience, and regeneration. The existing reference genome is, however, highly fragmented into 47,877 scaffolds, hampering chromosome-scale analyses for these fields. Here, we report a high-quality, chromosome-scale genome assembly for the white-eyed mutant strain of this cricket, generated using a combination of Nanopore and PacBio HiFi long reads, integrated with Hi-C da
ABSTRACT Mitochondrial autophagy (mitophagy) is an essential and evolutionarily conserved process that maintains mitochondrial integrity via the removal of damaged or superfluous mitochondria in eukaryotic cells. Phosphatase and tensin homolog (PTEN)-induced putative kinase 1 (PINK1) and Parkin promote mitophagy and function in a common signaling pathway. PINK1-mediated ubiquitin phosphorylation at Serine 65 (Ser65-pUb) is a key event in the efficient execution of PINK1/Parkin-dependent mitophag
Abstract Cannabinoid receptor type 1 (CB1R) is the key modulator of neuronal viability. In particular, CB1R antagonists provide neuroprotective effects on neurotoxicity caused by e.g. neuronal injury. However, the underlying mechanisms and potential limitations of CB1R antagonism remain unclear. Here we investigated the impact of environmental conditions on CB1R antagonist effects. Unexpectedly, we have found that cell-permeable CB1R antagonists, rimonabant and AM251, induced significant cell de
Abstract Maternal perception of environmental conditions can direct offspring developmental trajectories, providing adaptive flexibility across taxa. In the band-legged ground cricket Dianemobius nigrofasciatus , maternal exposure to short days induces embryonic diapause at the cellular blastoderm stage in offspring. Here, we investigate molecular mechanisms underlying this transgenerational adaptation through genome assembly (1.45 Gbp) and time-series transcriptomic analyses of diapause and non
20,768 candidate protein sequences of <i>T. occipitalis</i>
Structural and functional annotation of Asian field cricket <i>T. occipitalis</i> gene model
GTF file of 20,768 candidate protein-coding gene of <i>T. occipitalis</i>
Abstract Cerebral organoids are three-dimensional in vitro cultured brains that mimic the function and structure of the human brain. One of the major challenges for cerebral organoids is the lack of functional vasculature. Without perfusable vessels, oxygen and nutrient supplies may be insufficient for long-term culture, hindering the investigation of the neurovascular interactions. Recently, several strategies for the vascularization of human cerebral organoids have been reported. However, the
Asian Edible cricket <i>Teleogryllus occipitalis</i> genome
