포항공과대학교 · Biochemistry, Genetics and Molecular Biology
Kyo Han Ahn 교수의 연구실은 주로 광학적 센서 및 형광 프로브를 중심으로 생물학적 분자 인식과 생체 이미징 기술을 개발하고 있습니다. 특히 이온(예: 시아니드 이온)과 금속 나노입자(금, 은)를 타겟으로 한 선택적 감지 기반의 형광 프로브 설계에 초점을 맞추고 있으며, 반응 메커니즘과 분자 상호작용을 통해 유도되는 형광 신호 변화를 정밀하게 제어하는 데에 기여하고 있습니다. 또한 두광자 형광 이미징 기술을 활용한 깊은 조직에서의 고해상도 생체 이미징 기술 개발도 진행 중입니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Chemical probes are valuable tools for the investigation of biochemical processes, diagnosis of disease markers, detection of hazardous compounds, and other purposes. Therefore, the development of chemical probes continues to grow through various approaches with different disciplines and design strategies. Fluorescent probes have received much attention because they are sensitive and easy-to-operate, in general. To realize desired selectivity toward a given analyte, the recognition site of a flu
Synthesis, optical properties, and applications of various types of benzocoumarin compounds are overviewed.
Fluorescence signaling in anion binding is modulated from quenching to enhancement by intramolecular H-bonding stabilization of anion-ionophore adducts; the intramolecular H-bonding is suggested to suppress the quenching processes otherwise possible and increase the conformational rigidity of the anionic adducts, leading to fluorescence enhancement in a selective fashion towards cyanide ion, among the various anions examined.
Besides the noble physical appearance of gold and silver, their novel chemical properties attracted the modern technology for various industrial, chemical and biological uses including medical applications. The widespread use of gold and silver, however, can cause potential hazards to our environment. Therefore, suitable detection methods are a prerequisite for the evaluation of their harmful effects as well as for studying their beneficial biological properties. Due to the several advantages ov
Fluorescent probes are essential tools for studying biological systems. The last decade has witnessed particular interest in the development of two-photon excitable probes, owing to their advantageous features in tissue imaging compared to the corresponding one-photon probes. This review summarizes various types of two-photon probes that have been applied to bioimaging, categorized by the principles in the probe design and the target analytes, which would provide a basis for the future developme
The fluorescence imaging of tissue is essential for studying biological events beyond the cellular level. Two-photon microscopy based on the nonlinear light absorption of fluorescent dyes is a viable tool for the high resolution imaging of tissue. A key limitation for deep tissue imaging is the autofluorescence from intrinsic biomolecules. Here, we report a systematic study that discloses relative autofluorescence interference, which is dependent on the type of tissue and the excitation and emis
A fluorescein-based fluorescent probe displays fluorescence enhancement for palladium species in the typical oxidation states of 0, +2 and +4 and is applied to monitor accumulated palladium in living organisms.
A highly sensitive fluorescent turn-on probe specific for methylmercury species as well as inorganic mercury ions has been developed on the basis of mercury ion-promoted hydrolysis of a fluorescein-derived aryl vinyl ether.
The promising features of fluorescence spectroscopy have inspired a quest for fluorescent probes for analysis and monitoring of molecular interactions in biochemical, medical, and environmental sciences. To overcome the competitive supramolecular interactions in aqueous media encountered with conventional molecular-recognition-based probes, the use of reaction-based probes that involve making or breaking of covalent bonds has emerged as a complementary sensing strategy to realize higher selectiv
Substituents can induce dramatic changes in the photoluminescence properties of N,O-chelated boron complexes. Specifically, the boron complexes of 2-(benzothiazol-2-yl)phenols become bright deep blue- and orange-red-emitting materials depending on amino substituents at the 5- and 4-positions of 2-(benzothiazol-2-yl)phenol, respectively. Absorption and emission data show that the resulting boron complexes have little or small overlap between the absorption and emission spectra and, furthermore, X
Vesicles exchange their contents through membrane fusion processes, kiss-and-run and full-collapse fusion. Indirect observation of these fusion processes using artificial vesicles enhanced our understanding on the molecular mechanisms involved. Direct observation of the fusion processes in a real biological system, however, remains a challenge owing to many technical obstacles. We report a ratiometric two-photon probe offering real-time tracking of lysosomal ATP with quantitative information for
Hidden nuggets of gold: Mono- and divinylgold complexes (see scheme), key intermediates in the gold-mediated cyclization reaction of N-(propargyl)benzamides, are characterized by NMR and X-ray diffraction analyses. The monovinylgold intermediates undergo proto-deauration in acetonitrile by the substrate. In aqueous media, they produce oxidized products. The divinylgold species undergo reductive elimination to produce the corresponding dimerized products.
A fluorescent probe that enables ratiometric imaging of endogenous hypochlorous acid (HOCl) in cells and tissues by two-photon microscopy is developed based on a red-emitting acetyl-benzocoumarin (AcBC) dye. An oxathiolane group in the probe reacts with HOCl to generate the AcBC dye, which involves a ratiometric fluorescence change only toward HOCl along with high sensitivity.
Alzheimer's disease (AD) is the most common form of dementia. The pathogenesis of the disease is associated with aggregated amyloid-β, hyperphosphorylated tau, a high level of metal ions, abnormal enzyme activities, and reactive astrocytes. This outlook gives an overview of fluorescent small molecules targeting AD biomarkers for ex vivo and in vivo imaging. These chemical imaging probes are categorized based on the potential biomarkers, and their pros and cons are discussed. Guidelines for desig
A heteroditopic receptor having crown ether and trifluoroacetylcarboxanilide groups selectively recognizes both potassium and cyanide ions in acetonitrile with an association constant of as high as Ka = 1.9 x 10(7) M(-1) through a highly cooperative ion-pair interaction, resulting in two orders of magnitude enhancement in the binding affinity.
