Madoka Suzuki 교수

Tetragonal barium titanate nanoparticles (BTNPs) have been exploited as nanotransducers owing to their piezoelectric properties, in order to provide indirect electrical stimulation to SH-SY5Y neuron-like cells. Following application of ultrasounds to cells treated with BTNPs, fluorescence imaging of ion dynamics revealed that the synergic stimulation is able to elicit a significant cellular response in terms of calcium and sodium fluxes; moreover, tests with appropriate blockers demonstrated tha

We fabricated fluorescent nanoparticles which monitor temperature changes without sensitivity to pH (4-10) and ionic strength (0-500 mM). The nanothermometers spontaneously enter living HeLa cells via endocytosis, enclosed in acidic organelles, i.e., endosome/lysosome, and then transported along microtubules in a temperature-dependent manner, working as "walking nanothermometers".

This short review begins with a brief introductory summary of luminescence nanothermometry. Current applications of luminescence nanothermometry are introduced in biological contexts. Then, theoretical bases of the "temperature" that luminescence nanothermometry determines are discussed. This argument is followed by the 10⁵ gap issue between simple calculation and the measurements reported in literatures. The gap issue is challenged by recent literatures reporting single-cell thermome

We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca(2+) burst.

We demonstrate that an increase in mitochondrial pH is implicated as an early event in adrenergically stimulated BAs. We further suggest that this pH increase may play a role in the potentiation of thermogenesis.

Could enzymatic activities and their cooperative functions act as cellular temperature-sensing systems? This review introduces recent opto-thermal technologies for microscopic analyses of various types of cellular temperature-sensing system. Optical microheating technologies have been developed for local and rapid temperature manipulations at the cellular level. Advanced luminescent thermometers visualize the dynamics of cellular local temperature in space and time during microheating. An optica

Functionalized diamond nanocrystals persistently expand their use for sensing and labeling in a biological context. The surface of such crystals modified chemically adds additional modality to such applications. In this Perspective, we discuss mainly applications in nanothermometry but begin with a brief general introduction of fluorescent nanodiamonds. Then we consider temperature at the sub-cellular environment, explain the working principle of fluorescent nanodiamonds as temperature probes, a

Biochemical reactions in cells serve as the endogenous source of heat, maintaining a constant body temperature. This process requires proper control; otherwise, serious consequences can arise due to the unwanted but unavoidable responses of biological systems to heat. This review aims to present a range of responses to heat in biological systems across various spatial scales. We begin by examining the impaired thermogenesis of malignant hyperthermia in model mice and skeletal muscle cells, demon

Polyvinylalcohol (PVA) hydrogel containing both polyacrylic acids (PAA) and polyallylamines (PAlAm) has been investigated for some time. In this report the performances of two kinds of improved materials are shown. One was made with a very narrow gap mold, and the other by applying uniaxial stretching during gelation to realize an anisotropic hydrogel. As a result, thin films of 10- mu m thickness have been realized. These films are able to contract within 0.2 s under loading from 0 to 2 kg/cm/s