The University of Tokyo · 화학
마코토 츄노 교수의 연구실은 생체 분자, 특히 티올기와 카테콜아민을 중심으로 한 고감도 분석 기법을 개발하고 있습니다. HPLC-유도형 발광 및 전기화학적 검출 기반의 정밀 분석법을 활용해 생체 내 신경전달물질 및 효소 활성을 정량하며, 고혈압 모델 생쥐를 통한 신경조절 메커니즘 규명에도 주력하고 있습니다. 특히 카테콜-O-메틸트랜스퍼라제(COMT)의 생리적 기능과 병리적 역할에 대한 기초 연구가 핵심입니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
A method for analyzing biothiols based on high-performance liquid chromatography (HPLC)-fluorescence detection under hydrophilic interaction chromatography (HILIC) conditions has been developed. Thiols were derivatized with nonfluorescent ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F), which selectively reacts with the thiol groups to furnish the corresponding fluorescent SBD-thiols. Among the six different kinds of HILIC columns examined, the ZIC-HILIC column with sulfobetaine group
A simple method for the determination of catecholamines (norepinephrine, epinephrine, and dopamine) in urine is described. The analytes were extracted from urine by extraction using a monolithic silica disk-packed spin column. The spin column has a phenylboronate moiety, which forms a stable negatively charged complex with cis-hydroxyl groups of catecholamines. The extraction recoveries of catecholamines were above 92.2%. The extracted catecholamines were separated on a reversed-phase column fol
We have previously reported a highly sensitive method for the measurement of catechol-O-methyltransferase (COMT) activities in rat erythrocytes with norepinephrine (NE), an endogenous native substrate, using high-performance liquid chromatography (HPLC)-fluorescence or peroxyoxalate chemiluminescence reaction detection. Applying this method to COMT activities in rat liver and kidney, known to have the highest activities of all organs, the optimum reaction conditions were investigated. Under the
We have previously reported that methylation of catecholamines by catechol-O-methyltransferase (COMT) was attenuated in spontaneously hypertensive rats (SHR) with acute hypotension as compared with that of Wistar-Kyoto (WKY) rats. Here we examined the soluble (S-) and membrane-bound (MB-) COMT activities and COMT protein in the liver, kidney, and erythrocytes in both strains. Both the activities and the amounts of MB-COMT in the liver were lower in SHR than in WKY rats, but no such trend was fou
Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3, 6-tri-O-methyl)-beta-cyclodextrin(TM-beta-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-beta-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 nM for each enantiomer. T
