Man Bock Gu
고려대학교 생명과학부 · 생화학·유전·분자생물학
Man Bock Gu 교수의 연구실은 나노소재와 생체분자 간의 상호작용을 중심으로 한 혁신적 생분석 기술 개발에 주력하고 있습니다. 특히 그래핀 옥사이드 기반의 레이블 프리 아ptamer 선별 기술과 나노입자에 의한 세포 독성 메커니즘 규명을 핵심 연구 방향으로 삼고 있으며, 이는 친환경 에너지 및 생체의료 분야의 기초 기술 발전에 기여하고 있습니다. 또한, 효소 고정화 기술을 통해 지속 가능한 바이오 프로세스 개발에도 기여하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Every cell has a silver lining! The toxicity of Ag nanoparticles is investigated using a panel of recombinant bioluminescent bacteria. The presence of the nanoparticles leads to the production of a superoxide radical (see figure). Furthermore, the Ag nanoparticles damage the cellular membranes, causing a disruption in the ion efflux system. Thus, the cells cannot effectively extrude the Ag ions and, hence, Ag nanoparticles cause more damage than do Ag ions. Supporting information for this articl
Immobilization is a key technology for successful realization of enzyme‐based industrial processes, particularly for production of green and sustainable energy or chemicals from biomass‐derived catalytic conversion. Different methods to immobilize enzymes are critically reviewed. In principle, enzymes are immobilized via three major routes (i) binding to a support, (ii) encapsulation or entrapment, or (iii) cross‐linking (carrier free). As a result, immobilizing enzymes on certain supports can e
Graphene oxide (GO) has the ability to separate free short ssDNA in heterogeneous solution. This feature is applied as a label free platform for screening of aptamers that bind to their target with high affinity and specificity. Herein, we report an aptamer selection strategy for Nampt protein based on GO.
A shortened 8-mer ssDNA aptamer was successfully truncated for four different tetracyclines with high affinity. The ultrasensitive colorimetric detection of oxytetracycline using this shortened aptamer was possible, which was about 500-fold enhanced compared to that obtained using the original 76-mer aptamer.
