The University of Tokyo · 생화학·유전·분자생물학
마사루 타노쿠라 교수의 연구실은 주로 생물학적 분자 간의 상호작용과 구조 기반 기능 해석을 중심으로 연구를 진행하고 있습니다. 특히 단백질-단백질 상호작용, 예를 들어 트로포닌 시스템 내에서 트로포닌 T, 트로포닌 C, 트로포닌 I 등 간의 결합 메커니즘을 생화학적 및 물리화학적 방법을 통해 분석합니다. 또한 NMR 분광법을 활용해 펩타이드 및 자연물 복합체의 구조와 산화환원 거동을 정량적으로 규명하는 데도 전문성을 기르고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Abstract The nmr titration curves of chemical shifts versus pH were observed for the protons of various histidine‐containing di‐ and tripeptides. With these results, the macroscopic p K a values and the chemical shifts intrinsic to each ionic species were determined by a computer curve‐fitting based on a simple acid dissociation sequence. The p K a value of the imidazole ring in N ‐acetyl‐ L ‐histidine methylamide was assumed to represent the intrinsic (or unperturbed) p K a of the imidazole rin
The binding of the chymotryptic troponin T subfragments to tropomyosin, troponin I, and troponin C was semiquantitatively examined by using affinity chromatography, and also by co-sedimentation with F-actin and polyacrylamide gel electrophoresis in 14 mM Tris/90 mM glycine. Circular dichroism spectra of the subfragments were measured to confirm that the subfragments retained their conformational structures. Based on these results, the binding sites of tropomyosin, troponin I, and troponin C on t
A complex mixture analysis by one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy was carried out for the first time for the identification and quantification of organic compounds in green coffee bean extract (GCBE). A combination of (1)H-(1)H DQF-COSY, (1)H-(13)C HSQC, and (1)H-(13)C CT-HMBC two-dimensional sequences was used, and 16 compounds were identified. In particular, three isomers of caffeoylquinic acid were identified in the complex mixture without any separation. In
