Kyoto University · 생화학·유전·분자생물학
마사요시 츠쿠하라 교수의 연구실은 인도플라립로텐트 줄기세포(iPSC)의 생존과 분화 조절 메커니즘을 중심으로, 세포 외기질과 수용체 간의 상호작용, 특히 라미닌-511/α6β1 인테그린과 E-카드헤린 간의 신호전달 경로에 초점을 맞추고 있습니다. 특히 자가화이팅(iPSC) 치료를 위한 3차원 밀폐형 배양 시스템 개발과 함께, 식물 및 동물 줄기세포의 재생 능력 분석을 통해 세포 기반 치료의 기초 기술을 확립하고 있습니다. 연구는 임상적 응용에 적합한 세포 배양 기술과 생체 적합성 기반의 표면 코팅 기술 개발로 이어지고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
This study was supported by a research center network for the realization of regenerative medicine of the Japan Agency for Medical Research and Development (AMED) under grant number JP20bm0104001h0108.
In human induced pluripotent stem cells (hiPSCs), laminin-511/α6β1 integrin interacts with E-cadherin, an intercellular adhesion molecule, to induce the activation of the phosphatidylinositol 3-kinase (PI3K)-dependent signaling pathway. The interaction between laminin-511/α6β1 integrin and E-cadherin, an intercellular adhesion molecule, results in protection against apoptosis through the proto-oncogene tyrosine-protein kinase Fyn(Fyn)-RhoA-ROCK signaling pathway and the Ras homolog gene family m
Genetic analysis of plant regeneration ability in rice was studied in 14 x 14 diallel crosses using 4 japonica, 4 javanica and 6 indica varieties. Calli were obtained from mature seeds and regenerated plantlets were induced in suspension cultures. Among the 14 varities, Kele showed the highest regeneration ability. The largest number of regenerated plantlets was obtained in the F1 hybrid of Kele × Banten. No regenerated plantlet was observed in 4 parents and 8 Fls. The relationship between Vr an
As autologous induced pluripotent stem cell (iPSC) therapy requires a custom-made small-lot cell production line, and the cell production method differs significantly from the existing processes for producing allogeneic iPSC stocks for clinical use. Specifically, mass culture to produce stock is no longer necessary; instead, a series of operations from iPSC production to induction of differentiation of therapeutic cells must be performed continuously. A three-dimensional (3D) culture method usin
It was previously believed that human induced pluripotent stem cells (hiPSCs) did not show adhesion to the coating material Laminin 221, which is known to have specific affinity for cardiomyocytes. In this study, we report that human mononuclear cell-derived hiPSCs, established with Sendai virus vector, form peninsular-like colonies rather than embryonic stem cell-like colonies; these peninsular-like colonies can be passaged more than 10 times after establishment. Additionally, initialization-de
We reported in 2018 that among several extracellular matrices, fibronectin, type I collagen, type IV collagen, laminin I, fibrinogen, and bovine serum albumin, fibronectin is particularly useful for adhesion of porcine pancreatic tissue. Subsequently, we developed a technology that enables the chemical coating of the constituent motifs of fibronectin onto cell culture dishes. In this experiment, we used islets (purity ≥ 90%), duct epithelial cells (purity ≥ 60%), and acinar cells (purity ≥ 99%)
The rate of cell proliferation is a crucial factor in cell production under good manufacturing practice (GMP) control. In this study, we identified a culture system for induced pluripotent cells (iPSCs) that supports cell proliferation and viability and maintains the cells in an undifferentiated state even at 8 days after seeding. This system involves the use of dot pattern culture plates that have been coated with a chemically defined scaffold which has high biocompatibility. Under cell starvat
